Abstract: Objective： To observe the effect of avidin-biotin binding system(ABBS) to the adhesion between seed cells and scaffolds in bone tissue engineering, and investigate the role of ABBS in bone tissue engineering. Methods： We used adipose-derived stem cells (ADSCs) as seed cells and β-tricalcium phosphate (β-TCP) as scaffolds to establish tissue engineering bone, which was modified by ABBS. We used immunofluorescence and flow cytometry to test the efficiency of biotinylated ADSCs. The materials were divided into ABBS group (ADSCs and β-TCP modified by ABBS) and control group, and we tested the adhesion efficiency of both groups. We used scanning electron microscopy to detect the biocompatibility of ADSCs with the porous β-TCP scaffolds (control group) and the ABBS-modified porous β-TCP scaffolds (experimental group). Results： Biotinylated ADSCs staining showed that the biotin binding sites were in the cell cytoplasm, and flow cytometry showed that the rate of biotinylated positive cells was 95%. At different time points (10 min, 30 min, 60 min, 12 h, 24 h), the adhesion rates of control group vs ABBS group were (2.31±0.14)% vs (21.75±4.69)%, (11.96±2.53)% vs (54.82±12.37)%, (33.48±9.51)% vs (78.69±15.65)%, (78.29±10.63)% vs (95.46±7.38)%, and (94.79±10.42)% vs (98.13±1.45)%, respectively. The adhesion rate of the ABBS group during the early stages (10 min, 30 min, 60 min, 12 h) were much higher. There was no much difference between the two groups under scanning electron microscopy. Conclusions： ABBS can promote the adhesion of ADSCs on scaffolds during the early stage, which is good for cell proliferation, and has no obvious effect on the biocompatibility of tissue engineered bone.