Abstract: Objective： Type of human papillomavirus (HPV) points detection value in the diagnosis of cervical lesions. Methods： Selection in August 2013-January 2015 in xuhui dahua outpatient women cervical cancer screening, age less than 25 years old, a cerical liquid thin-layer cytology test (TCT), fluorescence PCR detection of HPV for nucleic acid amplification, divided into two groups, HPV01, 35, 52, 53, 58 (16); A total of 298 cases of HPV02, 31, 33, 45, 56 (18).There are indications of selected 182 patients with abnormal transformation zone positioning biopsy under colposcope, pathological examination, including 15 routine cervical Loop electricity cut method (Loop electrosurgieal excision procedure, LEEP), take its pathological changes is more serious for the final pathologic diagnosis. Results： In this study based on acuity CIN lesions Ⅱ for pathology is positive, with the lesion degree CIN I for pathology or less negative, HPV is used for screening or greater CIN Ⅱ lesion degree of sensitivity, specific, positive predictive value and negative predictive value, respectively, 93.33%, 84.43%, 35%, 99, 3 %.Positive HPV01 group 25 cases, positive HPV02 group 15 cases (with 5 positive HPV01 group) at the same time, the negative group 142 cases.In CIN or Ⅱ lesions (a total of 15 cases), 9, 5, and 1 case respectively, compared the risk of cancer, difference of three groups was statistically significant (χ²= 93.98, P< 0.01).For cervical intraepithelial neoplasia acuity CIN Ⅱ lesions of 15 cases, TCT testing positive rate was 53.33% (8/15), HPV detection positive rate was 93.33% (14/15). Conclusions： TCT joint HPV detection, can identify patients with cervical lesions of higher risk CINII or, more should pay attention to the role of HPV clinically.