Abstract: Objective：To summarize the preliminary experience suprapubic transvesical single-port robot assisted radical prostatectomy for prostate cancer. Methods：Three patients with prostate cancer were selected to undergo single-port robot assisted laparoscopic radical prostatectomy via an suprapubic transvesical approach. A single-port channel was established and was introduced percutaneously into the bladder under direct vision. The surgical procedure included incision of the bladder neck along the border of the prostate, separation and removal of the vas deferens, seminal vesicles, and prostate ligaments outside the prostate capsule. The dorsal vein complex (DVC) was not sewed. The prostate tip was cut off and the urethra was cut. The urethral bladder neck anastomosis was performed. Results：All 3 cases were performed with single-port robot assisted laparoscopic surgery via the suprapubic transvesical approach. The volume of the prostate was 16 mL, 22 mL, and 43 mL, respectively, and the operation time was 90 min, 120 min, and 150 min, respectively. The intraoperative blood loss was 50 mL, 150 mL, and 200 mL, respectively. The wound drainage tube and bladder stoma were not placed and the catheters were removed 7-9 days after surgery. No patients developed urinary incontinence after removing the catheter. Conclusions：Single-port robot assisted laparoscopic radical prostatectomy via the suprapubic transvesical approach is safe and effective for early stage prostate cancer, and it is worthy of further promotion.