Abstract: Objective：To explore the clinical value of interventional ultrasound in the diagnosis and treatment of acquired immunodeficiency syndrome (AIDS)-related liver diseases. Methods：From April 2014 to April 2018, 55 patients with AIDS related liver disease underwent ultrasound-guided biopsy, puncture, and catheter drainage in our institute were selected. The diagnostic compliance rate, curative rate, and complications were observed and calculated. Results：During the ultrasound-guided biopsy in 18 cases with solid hepatic lesions and four cases with diffuse hepatomegaly, 16 malignancies and 6 benignities were pathologically proved, with the diagnostic compliance rate being 100%. In the 33 cases with 48 cystic and solid-cystic lesions, the puncture treatment included 15 puncture pumping pus and 33 catheter drainage, with 31 being cured and two being improved. Complication was observed in only one patient, that is nausea after catheterization for liver abscess and without treatment. Conclusions：Interventional ultrasound is a minimally invasive, safe, convenient, and effective method for the diagnosis and treatment of AIDS-related liver diseases with low treatment cost. Moreover, medical staff are safe to conduct the manipulations. It is a preferred diagnostic and treatment method worthy of promotion.