MicroRNA-29b表达改变对卵巢癌细胞增殖及侵袭的影响
The role of microRNA-29b in ovarian cancer cell proliferation and invasion and its mechanism
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摘要: 目的：探讨microRNA-29b (miRNA-29b)在卵巢癌细胞中的表达差异及其对卵巢癌细胞增殖和侵袭能力的影响。方法：采用real-time PCR技术检测人卵巢癌上皮细胞系SKOV-3及正常人卵巢上皮细胞系IOSE80中miRNA-29b的表达水平；采用转染技术上调SKOV-3细胞中miRNA-29b的表达水平；采用CCK-8法检测SKOV-3细胞增殖能力；采用Transwell法检测SKOV-3细胞的侵袭能力；采用Western印迹法检测SKOV-3细胞中蛋白表达水平。结果：与IOSE80细胞相比，人卵巢癌上皮细胞系SKOV-3细胞miRNA-29b的表达下降（P<0.05）。转染miRNA-29b mimic后SKOV-3细胞中miRNA-29b的表达水平增加；从第4天开始，转染miRNA-29b的SKOV3细胞增殖能力下降（P<0.05）。转染miRNA-29b减少了通过聚碳酸酯膜的SKOV3细胞数量，抑制了SKOV3细胞的侵袭能力（P<0.05）；转染miRNA-29b mimic后，卵巢癌细胞SKOV3中抑制凋亡蛋白Bcl-2和侵袭相关蛋白MMP-9的表达均降低，与阴性对照组相比差异有统计学意义（P<0.05）。结论：miRNA-29b可通过抑制凋亡相关蛋白Bcl-2和侵袭相关蛋白MMP-9的表达来抑制卵巢癌细胞的增殖和侵袭。Abstract: Objective：To investigate the expression of miRNA-29b in ovarian cancer cells and its effects on proliferation and invasion of ovarian cancer cells and the underlying mechanisms. Methods：Real-time RT-PCR was used to detect the expression level of miRNA-29b in human ovarian cancer epithelial cell line SKOV-3 and human normal ovarian epithelial cell line IOSE80. The expression of miRNA-29b in SKOV-3 cells was up-regulated by transfection technique. CCK-8 assay was used to detect the proliferation of SKOV-3 cells. Transwell migration assay was used to detect the invasion of SKOV-3 cells. Western blotting was used to detect the level of protein expression in SKOV-3 cells. Results：The expression of miRNA-29b in human ovarian epithelial cell line SKOV-3 cells decreased significantly (compared with IOSE80 cells, P<0.05). The expression level of miRNA-29b in SKOV-3 cells was increased significantly after miRNA-29b mimic transfection (P<0.05). From the 4th day, the proliferation of miRNA-29b-transfected SKOV3 cell was significantly increased (P<0.05). At the same time, transfection of miRNA-29b significantly reduced the number of SKOV3 cells through the polycarbonate membrane and inhibited the invasiveness of SKOV3 cells (P<0.05). After transfection of miRNA-29b mimic, the expression of Bcl-2 and the invasion-related protein MMP-9 in the ovarian cancer cell line SKOV3 were significantly lower than those in the negative control group (P<0.05). Conclusions：miRNA-29b can inhibit the proliferation and invasion of ovarian cancer cells, probably via inhibiting the expression of apoptosis related protein Bcl-2 and invasion related protein MMP-9.
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Keywords:
- miRNA-29b /
- ovarian cancer /
- cell proliferation /
- cell invasion /
- regulation mechanism
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