Abstract: Objective：To investigate the feasibility, safety and efficacy of a novel endoscopic multi-functional snare for endoscopic resection of gastrointestinal lesions in a porcine model. Methods：The new endoscopic multi-function snare was used for endoscopic resectionin porcine stomach and porcine intestine in vitro or in vivo, respectively. Under condition of different electrical setting (20 W, 30 W, 40W), the stimulated resected lesions with diameter of about 1 cm were excised, followed by argon plasma coagulation for the wound.The complete resection rate, wound healing 1 week after resection(in vivo experiment), intraoperative and perioperative complications were observed and recorded.The resected lesions and the wounds were sent for pathological examination to observe the muscularis propria injury. Results：It was shown that all the 9 simulated lesions in the stomach were completely resected in vitro.The resection effect in the intestine in vitro was the best under the low power (20 W), while the escharosis of the intestinal mucosa was obvious under higher voltage settings, so low voltage setting was used in the further experiment in vivo. No muscularis propria injury was observed macroscopically and pathologically in vitro. In gastric surgery in vivo, all the 9 lesions were completely resected and no perforation or bleeding on the wound was observed 1 week after operation. In intestinal surgery in vivo, all the 15 lesions were completely resected, and healing of different degree was observed, no complication was found on the wound, muscularis propria damage was observed in 1 case (6.7%, 1/15) 1 week after operation. Conclusions：The new multi-functional snare is safe and effective in endoscopic resection in porcine model in vitro and in vivo. It is recommended to set the low power of 20 W during intestinal surgery.