Abstract: Objective：To study the value of micro-CT in monitoring the dynamic evolution of acute pulmonary tuberculosis in mice. Methods：Establish the murine tuberculosis model of C57BL/6J in intranasal as the experiment group. The control group was set at the SPF level. Micro-CT scans were undertaken 1, 4, 8, 12 weeks later， respectively. And then the mice were sacrificed and the lung tissue was taken for pathological analysis. Results：In the experiment group, nine mice died and three were negative. So, totally 28 mice were enrolled. There were 7,6,8,7 mice in each subgroup, respectively. One week after infection, ground glass opacity (GGO) was 71.4% and consolidation was 57.1% in the experiment group. Corresponding pathology showed alveolar exudate and alveolar septum widened. Four weeks after infection, the imaging findings included GGO and patchy opacities (in 66.7%) in the experiment group. Microscopically, alveolar effusion and consolidation, with epithelioid cells, multinucleated giant cells and foam cells were found. Eight weeks after infection, nodules, air bronchogram and consolidation had equal proportion in the experiment group. Microscopically, it showed consolidation and tuberculosis nodules. 12 weeks after infection, nodules had the highest proportion, about 57.1% in the experiment group. Microscopically, the nodules showed granulomas. Conclusions：The images of acute pulmonary tuberculosis murine model can reflect the pathological changes, which can be used to simulate the course of human pulmonary tuberculosis and evaluate the disease process.