Abstract: Objective: To explore the positive rate of detecting helicobacter pylori(Hp) infection in different groups regarding gender, age and site of biopsy. Methods: Immunohistochemistry staining was conducted by EnVision two-step method for biopsy specimens from 2131 clinical patients in Beijing, and the positive rate of detecting Hp infection was evaluated regarding gender, age and site of biopsy, respectively. Results: The method of immunohistochemistry EnVision showed characteristics including clear background, stable quality and standard procedure for detecting Hp infection in gastroscopic biopsy. In 2131 patients, the overall positive rate of detecting Hp infection in 2131 gastroscopic biopsy cases in Beijing was 35.6%. The positive rate was 40.9% in male group while it was 30.5% in female group. There were significant differences regarding Hp infection positive rate of males among groups with different age level, and the infection rate in males younger than 30 years was the highest (60.86%, P＜0.05). There was no significant difference regarding positive rate of females among groups with different age level (P>0.05). On each age level, the positive rate in males was higher than that in females (P＜0.05). The positive rate of Hp infection in the middle site of lesser curvature of stomach was the highest(36.0%), while that in greater curvature of gastric antrum was the lowest(24.0%), and the difference was significant(P＜0.05). Conclusions: Among the gastroscopic biopsy in Beijing, the Hp infection positive rate in males was higher than that in females. And the peak positive rate of infection occurred in male younger than 30 years. Furthermore, Hp infection was more common in the middle site of lesser curvature of stomach.