Abstract: Autophagy is involved in maintaining cell homeostasis as a highly conserved degradation pathway of intracellular metabolite and senescent or damaged organelles. Tumor-associated macrophages (TAMs) are the key cells in the tumor microenvironment, which can promote tumorogenesis, development, and metastasis through M2 polarization. Autophagy is an important component in the construction of the tumor microenvironment by regulating TAMs polarization and metabolism. This article reviews the mechanism of autophagy regulating the interaction between macrophages and tumor microenvironment, aiming to provide new potential theoretical autophagy regulatory targets for anti-tumor therapy.