宏基因二代测序技术对医院传染性疾病防控的价值

林佳冰; 高晓东; 胡必杰; 陈翔

doi:10.12025/j.issn.1008-6358.2020.20201747

宏基因二代测序技术对医院传染性疾病防控的价值

Value of metagenomic next-generation sequencing in prevention and control of nosocomial infectious diseases

摘要

摘要:
目的探讨宏基因二代测序（metagenomic next-generation sequencing，mNGS）技术对医院传染性疾病防控的价值。
方法回顾性分析2018年1月1日至2019年12月31日复旦大学附属中山医院收治的5例传染性疾病患者的病例资料，比较其常规微生物培养和mNGS结果。
结果 5例患者常规微生物培养均为阴性，mNGS结果：炭疽芽孢杆菌1例，霍乱弧菌1例，结核分枝杆菌复合群1例，三日疟原虫1例，恶性疟原虫1例。根据mNGS结果，提早确诊传染性疾病并调整医院感染防控措施，随访30 d内，未出现院内传播新发病例。
结论 mNGS在传染性疾病的早期识别与诊断中有重要作用，对于医院感染防控早期干预起到良好的指导效果。

Abstract:
Objective To explore the value of metagenomic next-generation sequencing (mNGS) in the prevention and control of nosocomial infectious diseases.
Methods Retrospectively analyzed the data of 5 patients with infectious diseases from January 1, 2018 to December 31, 2019 in Zhongshan Hospital, Fudan University. Compared the results of mNGS and routine microbial culture.
Results Among the 5 selected patients, routine microbial culture were all negative, while the mNGS detected Bacillus anthracis, Vibrio cholerae, Mycobacterium tuberculosis complex, Plasmodium vivax and Plasmodium falciparum, respectively. All the 5 patients were diagnosed with infectious diseases earlier by mNGS, and the control measures of nosocomial infection were adjusted according to the mNGS results. No new cases of nosocomial transmission were found within 30 days of follow-up.
Conclusions The mNGS plays an important role in the early identification and diagnosis of infectious diseases, and plays a good guiding effect for the early intervention of nosocomial infection prevention and control.

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