Radil在肝癌组织中的表达及对肝癌发展的影响

孙海香; 史颖弘; 樊嘉; 杨柳晓

doi:10.12025/j.issn.1008-6358.2020.20201091

Radil在肝癌组织中的表达及对肝癌发展的影响

Expression of Ras association and DIL domains (Radil) and its effect on hepatocellular carcinoma

摘要

摘要:
目的探讨肝癌组织中Radil（Ras association and DIL domains）的表达水平，并进一步分析其对肝癌发展的影响。
方法采用免疫组化和Western blot对手术切除的癌组织及对应的癌旁组织进行蛋白质水平检测；用Western blot方法分析各肝癌细胞系中Radil的表达，并利用敲低或过表达的方法分析Radil表达水平的变化对肝癌细胞生长、运动的影响。
结果 Radil在肝癌组织中表达均显著高于对应癌旁组织，高表达Radil可以促进肝癌细胞生长、迁移，而抑制凋亡相关分子的表达。
结论 Radil在肝癌组织中高表达，可显著促进肝癌细胞的生长、迁移，有望为肝癌的治疗和预后判断提供参考。

Abstract:
Objective To assess the expression level of Ras association and DIL domains (Radil), and its effect on hepatocellular carcinoma (HCC).
Methods The expression of Radil was measured by immunohistochemistry and Western blot in HCC tissue samples. Then the expression of Radil was measured in several HCC cell lines by Western blot, and Rnock-down and over-expression were carried out in HCC cell lines respectively to test cell proliferation and movement ability.
Results The expression of Radil in HCC tissues was significantly higher than that in corresponding adjacent tissues. The expression of Radil promoted HCC cell proliferation and migration ability, and the apoptosis-related protein expression levels were decreased.
Conclusions This study suggests that Radil is overexpressed in HCC. It may play an important role in carcinogenesis or aggression and may be a useful marker for the prognosis evaluation of HCC.

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