宏基因二代测序技术对脊柱感染病原学诊断的价值

金文婷; 李娜; 周晓岗; 张尧; 黄英男; 苏逸; 姚雨濛; 缪青; 马玉燕; 李冰; 王青青; 王萌冉; 骆煜; 蔡思诗; 潘珏; 胡必杰

doi:10.12025/j.issn.1008-6358.2020.20200987

宏基因二代测序技术对脊柱感染病原学诊断的价值

Etiological dignostic value of metagenomic next-generation sequencing in spinal infection

摘要

摘要:
目的探讨宏基因二代测序（metagenomic next-generation sequencing，mNGS）技术对脊柱感染病原学诊断的价值。
方法回顾性分析2018年1月至2019年12月复旦大学附属中山医院收治的24例疑似脊柱感染患者的病例资料，采用常规微生物培养法和mNGS技术检测脊柱组织标本。
结果 mNGS总体检测阳性率高于常规培养法（62.5%vs 35.0%），mNGS平均耗时36~48 h，明显短于常规培养法的平均天数21.8 d。脊柱感染主要检出病原体为结核分枝杆菌、金黄色葡萄球菌、大肠埃希菌。
结论 mNGS能够快速检测脊柱感染病原体，为临床早期精准治疗提供重要的病原学依据。

Abstract:
Objective To explore the value of metagenomic next-generation sequencing (mNGS) in the pathogen diagnosis of spinal infection.
Methods A retrospective analysis was performed in 24 hospitalized patients with spinal infection in Zhongshan Hospital, Fudan University from January 2018 to December 2019. Spinal tissues were detected by microbial culture method and mNGS.
Results The overall positive rate of mNGS was higher than that of culture method (62.5% vs 35.0%). The average detection time of mNGS was 36-48 h, significantly shorter than that of culture method (21.8 days). The main pathogens of spinal infection were Mycobacterium tuberculosis complex, Staphylococcus aureus, and Escherichia coli.
Conclusions The mNGS is effective in quick detection of spinal infection pathogens, which can provide important etiological basis for early precise treatment.

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