Objective To prepare high mobility group chromosal protein N2 (HMGN2) protein in vitro, and observe its capacity to enter into osteosarcoma cells and its cell localizations.

Methods After packaging HMGN2 overexpressing lentivirus, the recombinant lentivirus was delivered into HEK-293FT cells. HMGN2 protein was purified from the harvested 293FT cells using protein purification kit. Purified HMGN2 protein was used to treat cultured osteosarcoma cells. Whether the exogenous HMGN2 could enter into osteosarcoma cells and its cell localizations were detected by immunocytochemistry assay.

Results HMGN2 protein was successfully prepared and purified. The exogenous HMGN2 protein entered into osteosarcoma cells in culture, and mainly located in the nucleus.

Conclusions HMGN2 could be successfully prepared and purified, and can enter into cells. It mainly localizes in the nucleus, to lay a foundation for its biological effects.