室内空气污染对哮喘儿童血清miR-155水平的影响

王诗敏; 孙素静; 纪一伦

doi:10.12025/j.issn.1008-6358.2019.20191401

室内空气污染对哮喘儿童血清miR-155水平的影响

Effects of indoor air pollution on asthma and serum level of miR-155 in children

摘要

摘要:
目的探讨室内空气污染与儿童哮喘的关系及潜在的分子机制。
方法选择176名健康受试者作为对照组，176例哮喘患儿作为哮喘组。应用实时荧光定量PCR技术检测受试者血清miR-155水平。测量甲醛（HCHO）、NO₂和颗粒（PM₁₀、PM_2.5和PM₁）。通过单因素和多因素logistic回归分析，评价空气污染物与哮喘风险的关系。
结果哮喘组血清miR-155水平明显高于对照组（P < 0.001）。两组儿童过敏史、母乳喂养史、环境烟草烟雾史、PM_2.5、HCHO差异与儿童哮喘发作相关（P < 0.05）。哮喘组血清miR-155水平与室内PM_2.5、HCHO水平密切相关（P < 0.05），对照组无此相关性。
结论哮喘患儿血清miR-155水平与室内PM_2.5和HCHO水平密切相关。室内空气污染加重了西安地区儿童哮喘的发生，并诱导其miR-155水平变化。

Abstract:
Objective To explore the relationship between indoor air pollution and asthma in children and its potential molecular mechanism.
Methods A total of 176 healthy subjects were selected as control group and 176 asthmatic children as asthma group. The level of serum miR-155 was detected by real-time fluorescence quantitative polymerase chain reaction (PCR). HCHO, NO₂, and particles (PM₁₀, PM_2.5, and PM₁) were measured. Univariate and multivariate logistic regression analysis were used to evaluate the relationship between air pollutants and asthma risk.
Results The serum level of miR-155 in asthma group was significantly higher than that in control group (P < 0.001). Allergy history of Children, breastfeeding history, environmental tobacco smoke history, PM_2.5, and HCHO were related to the occured of asthma (P < 0.05). The level of serum miR-155 in asthma group was related to the levels of PM_2.5 and HCHO in the room (P < 0.05), and this relationship was not observed in the control group.
Conclusions The level of serum miR-155 is closely related to the levels of PM_2.5 and HCHO in children with asthma. Indoor air pollution aggravates the incidence of asthma in children in Xi'an province of China, and induces change of miR-155 level.

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