Abstract: Objective：To investigate the inhibitory effect of retinoic-acid-related orphan nuclear receptor C (RORC/RORγ) on the proliferation of Siha and HeLa cells and explore the possible mechanisms. Methods：The inhibitory effect of RORC on the proliferation of Siha and HeLa cells was evaluated by cell counting kit-8 (CCK8) assay. Glucose levels in Siha and HeLa cells were measured using glucose assay kit. The expression levels of RORC, glucose transporter 1 (GLUT1), lactate dehydrogenase A (LDHA), and hypoxia-inducible factor 1α (HIF-1α) were determined by Western blotting. Results：The results of CCK-8 showed that RORC significantly inhibited the proliferation of Siha and HeLa cells (P<0.05). Glucose metabolism test results showed that glucose uptake in RORC overexpression cells decreased (P<0.05). Western blotting results showed that the expressions of GLUT1, LDHA, and HIF-1α in RORC overexpression cells were downregulated markedly (P<0.05). Conclusions：RORC could inhibit the proliferation of the Siha and HeLa cervical cancer cell lines through inhibition of their glucose metabolism.