Abstract: Objective：To explore the spatial memory of aged mice after propofol anesthesia. Methods：Eighteen male C57BL/6 mice, aged 15 months, were randomly enrolled into the propofol anesthesia group and control group. The propofol anesthesia group was given an intraperitoneal injection of 250 mg/kg propofol, and the control group was given a 20% medium/long chain fat emulsion injection intraperitoneally. The rotarod experiment was performed to record the average speed of mouse movement before anesthesia. The radial water maze (RAWM) test was performed 24 h after the intraperitoneal injection for 2 consecutive days, and the number of errors that the mouse entered the wrong arm was recorded. The difference of errors in the five blocks on the second day between the two groups was compared. Results：There was no significant difference in the time of rotarod between the two groups. Three of nine mice in the propofol anesthesia group and the control group did not reach the learning standard in the radial water maze test, accounting for 30%. There was no significant difference of errors on the second day of the RAWM test between the propofol anesthesia group and the control group (P=0.089). Conclusions：Intraperitoneal injection of 250 mg/kg propofol has no significant effect on the shortterm spatial memory in 15month aged mice.