血管内皮祖细胞鉴定及其促血管新生机制的研究进展

潘天岳; 刘浩; 符伟国; 董智慧

doi:10.12025/j.issn.1008-6358.2019.20190351

血管内皮祖细胞鉴定及其促血管新生机制的研究进展

复旦大学附属中山医院血管外科，上海 200032

基金项目: 国家自然科学基金（81970407），上海市优秀学术带头人（19XD1401200），上海市卫生计生系统优秀学科带头人培养计划（百人计划，2018BR40），复旦大学附属中山医院人才培养计划优秀骨干计划（2015ZSYXGG02）.

计量
- 文章访问数: 1955
- HTML全文浏览量: 9
- PDF下载量: 811
出版历程
- 收稿日期: 2019-03-16
- 修回日期: 2019-05-28
- 刊出日期: 2019-08-24

Research progress on vascular endothelial progenitor cells and their mechanism of promoting angiogenesis

Funds: Shanghai Excellent Academic Leader (No. 19XD1401200); Shanghai Health Family Planning System Excellent Discipline Leader Training Plan (No. 2018BR40); National Natural Science Foundation Youth Fund Project (No. 30801122); Zhongshan Hospital Affiliated to Fudan University Talent Training Plan-Excellent Backbone Plan (No. 2015ZSYXG02)

摘要

摘要: 血管内皮细胞（endothelial cells, ECs）既往被认为在出生后血管新生中发挥主要作用。1997年Asahara等从外周血分离出表达CD34抗原的单个核细胞，即血管内皮祖细胞（endothelial progenitor cells，EPCs）。EPCs是一类具有促血管生成能力的干细胞总称，尚未发现特异性免疫表型，故主要根据具有形成血管的功能鉴定。目前一般认为，EPCs来源于骨髓和外周血管壁，其中能分化为ECs者被称为内皮集落形成细胞（endothelial colony forming cells，ECFCs）。当组织缺血或损伤时，来自骨髓的EPCs被组织释放的损伤信号动员后进行迁移和归巢。活化的EPCs通过旁分泌作用招募更多的局部ECFCs和ECs来形成微血管，进而促进组织修复。因此，对EPCs的深入研究有助于临床上治疗性血管新生相关研究的进行和方法的发展。
- 血管内皮祖细胞 /
- 内皮集落形成细胞 /
- 治疗性血管新生
Abstract: Endothelial cells (ECs) were considered to play a main role in postnatal angiogenesis until CD34+ mononuclear cells were isolated from peripheral blood by Asahara et al in 1997, and these cells were named as putative endothelial progenitor cells (EPCs). EPCs are a group of stem cells with angiogenic function, and have no specific immunophenotype. Identification of EPCs is mainly based on their vasculogenic ability. It is widely recognized that EPCs originate either from bone marrow or peripheral vascular wall. The cells which can differentiate to immature endothelial cells in vitro are called endothelial colony forming cells (ECFCs). Signals release from ischemic tissues mobilize the EPCs from bone marrow and guide them to migrate and home to the ischemic sites. The active EPCs recruit more resident ECFCs and ECs to form neomicrovessels and then promote the tissues repair. More intensive studies on the nature of EPCs will contribute to the development of clinical therapeutic angiogenesis research and methods.
- endothelial progenitor cells /
- endothelial colony forming cells /
- therapeutic angiogenesis

HTML全文

参考文献(47)

[1]	Risau W, Sariola H, Zerwes H G, et al. Vasculogenesis and angiogenesis in embryonic-stem-cell-derived embryoid bodies[J]. Development, 1988,102(3):471-478.
[2]	Jack L. Cronenwett K W J. Rutherford''s Vascular Surgery[M]. Saunders Elsevier, 2010.
[3]	Folkman J, Shing Y. Angiogenesis[J]. J Biol Chem, 1992,267(16):10931-10934.
[4]	Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis[J]. Science, 1997,275(5302):964-967.
[5]	Zhang S J, Zhang H, Wei Y J, et al. Adult endothelial progenitor cells from human peripheral blood maintain monocyte/macrophage function throughout in vitro culture[J]. Cell Res, 2006,16(6):577-584.
[6]	Peichev M, Naiyer A J, Pereira D, et al. Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors[J]. Blood, 2000,95(3):952-958.
[7]	Timmermans F, Plum J, Yoder M C, et al. Endothelial progenitor cells: identity defined? [J]. J Cell Mol Med, 2009,13(1):87-102.
[8]	Case J, Mead L E, Bessler W K, et al. Human CD34+AC133+VEGFR-2+ cells are not endothelial progenitor cells but distinct, primitive hematopoietic progenitors[J]. Exp Hematol, 2007,35(7):1109-1118.
[9]	Duda D G, Cohen K S, Scadden D T, et al. A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood[J]. Nat Protoc, 2007,2(4):805-810.
[10]	李国强, 刘珍珍, 孙晟轩, 等. 人脐血来源CD34+内皮祖细胞的分离、培养、鉴定及集落形成特性[J]. 中国免疫学杂志, 2013,29(6):575-579.
[11]	Ingram D A, Mead L E, Tanaka H, et al. Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood[J]. Blood, 2004,104(9):2752-2760.
[12]	Patel J, Donovan P, Khosrotehrani K. Concise Review: Functional Definition of Endothelial Progenitor Cells: A Molecular Perspective[J]. Stem Cells Transl Med, 2016,5(10):1302-1306.
[13]	Yoder M C, Mead L E, Prater D, et al. Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals[J]. Blood, 2007,109(5):1801-1809.
[14]	Sieveking D P, Buckle A, Celermajer D S, et al. Strikingly different angiogenic properties of endothelial progenitor cell subpopulations: insights from a novel human angiogenesis assay[J]. J Am Coll Cardiol, 2008,51(6):660-668.
[15]	Shantsila E, Watson T, Tse H F, et al. New insights on endothelial progenitor cell subpopulations and their angiogenic properties[J]. J Am Coll Cardiol, 2008,51(6):669-671.
[16]	Asahara T, Kawamoto A, Masuda H. Concise review: Circulating endothelial progenitor cells for vascular medicine[J]. Stem Cells, 2011,29(11):1650-1655.
[17]	Rehman J, Li J, Orschell C M, et al. Peripheral blood "endothelial progenitor cells" are derived from monocyte/macrophages and secrete angiogenic growth factors[J]. Circulation, 2003,107(8):1164-1169.
[18]	Sharpe E R, Teleron A A, Li B, et al. The origin and in vivo significance of murine and human culture-expanded endothelial progenitor cells[J]. Am J Pathol, 2006,168(5):1710-1721.
[19]	Harraz M, Jiao C, Hanlon H D, et al. CD34- blood-derived human endothelial cell progenitors[J]. Stem Cells, 2001,19(4):304-312.
[20]	Bailey A S, Willenbring H, Jiang S, et al. Myeloid lineage progenitors give rise to vascular endothelium[J]. Proc Natl Acad Sci U S A, 2006,103(35):13156-13161.
[21]	Moldovan N I. Role of monocytes and macrophages in adult angiogenesis: a light at the tunnel’s end[J]. J Hematother Stem Cell Res, 2002,11(2):179-194.
[22]	Ingram D A, Mead L E, Moore D B, et al. Vessel wall-derived endothelial cells rapidly proliferate because they contain a complete hierarchy of endothelial progenitor cells[J]. Blood, 2005,105(7):2783-2786.
[23]	Fang S, Wei J, Pentinmikko N, et al. Generation of Functional Blood Vessels froma Single c-kit+ Adult Vascular Endothelial Stem Cell[J]. PlosBiology,2012,10(10):e1001407.
[24]	Schniedermann J, Rennecke M, Buttler K, et al. Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels[J]. BMC Cell Biol, 2010,11:50.
[25]	Rapp B M, Saadatzedeh M R, Ofstein R H, et al. Resident Endothelial Progenitor Cells from Human Placenta Have Greater Vasculogenic Potential Than Circulating Endothelial Progenitor Cells from Umbilical Cord Blood[J]. Cell Med, 2012,2(3):85-96.
[26]	Schatteman G C, Hanlon H D, Jiao C, et al. Blood-derived angioblasts accelerate blood-flow restoration in diabetic mice[J]. J Clin Invest, 2000,106(4):571-578.
[27]	Hu Y, Davison F, Zhang Z, et al. Endothelial replacement and angiogenesis in arteriosclerotic lesions of allografts are contributed by circulating progenitor cells[J]. Circulation, 2003,108(25):3122-3127.
[28]	Bauer S M, Goldstein L J, Bauer R J, et al. The bone marrow-derived endothelial progenitor cell response is impaired in delayed wound healing from ischemia[J]. J Vasc Surg, 2006,43(1):134-141.
[29]	Iwakura A, Shastry S, Luedemann C, et al. Estradiol enhances recovery after myocardial infarction by augmenting incorporation of bone marrow-derived endothelial progenitor cells into sites of ischemia-induced neovascularization via endothelial nitric oxide synthase-mediated activation of matrix metalloproteinase-9[J]. Circulation, 2006,113(12):1605-1614.
[30]	Perry T E, Song M, Despres D J, et al. Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunction[J]. Cardiovasc Res, 2009,84(2):317-325.
[31]	Aicher A, Rentsch M, Sasaki K, et al. Nonbone marrow-derived circulating progenitor cells contribute to postnatal neovascularization following tissue ischemia[J]. Circ Res, 2007,100(4):581-589.
[32]	Urbich C, Aicher A, Heeschen C, et al. Soluble factors released by endothelial progenitor cells promote migration of endothelial cells and cardiac resident progenitor cells[J]. J Mol Cell Cardiol, 2005,39(5):733-742.
[33]	Miyamoto Y, Suyama T, Yashita T, et al. Bone marrow subpopulations contain distinct types of endothelial progenitor cells and angiogenic cytokine-producing cells[J]. J Mol Cell Cardiol, 2007,43(5):627-635.
[34]	Kawamoto A, Asahara T, Losordo D W. Transplantation of endothelial progenitor cells for therapeutic neovascularization[J]. Cardiovasc Radiat Med, 2002,3(3-4):221-225.
[35]	Sahoo S, Klychko E, Thorne T, et al. Exosomes from human CD34(+) stem cells mediate their proangiogenic paracrine activity[J]. Circ Res, 2011,109(7):724-728.
[36]	Valadi H, Ekstrom K, Bossios A, et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells[J]. Nat Cell Biol, 2007,9(6):654-659.
[37]	Goretti E, Rolland-Turner M, Leonard F, et al. MicroRNA-16 affects key functions of human endothelial progenitor cells[J]. J Leukoc Biol, 2013,93(5):645-655.
[38]	Bonauer A, Carmona G, Iwasaki M, et al. MicroRNA-92a controls angiogenesis and functional recovery of ischemic tissues in mice[J]. Science, 2009,324(5935):1710-1713.
[39]	Yoder M C, Ingram D A. The definition of EPCs and other bone marrow cells contributing to neoangiogenesis and tumor growth: is there common ground for understanding the roles of numerous marrow-derived cells in the neoangiogenic process? [J]. Biochim Biophys Acta, 2009,1796(1):50-54.
[40]	Basile D P, Yoder M C. Circulating and tissue resident endothelial progenitor cells[J]. J Cell Physiol, 2014,229(1):10-16.
[41]	Schniedermann J, Rennecke M, Buttler K, et al. Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels[J]. BMC Cell Biol, 2010,11:50.
[42]	Alvarez D F, Huang L, King J A, et al. Lung microvascular endothelium is enriched with progenitor cells that exhibit vasculogenic capacity[J]. Am J Physiol Lung Cell Mol Physiol, 2008,294(3): L419-L430.
[43]	Basile D P. The endothelial cell in ischemic acute kidney injury: implications for acute and chronic function[J]. Kidney Int, 2007,72(2):151-156.
[44]	Li D W, Liu Z Q, Wei J, et al. Contribution of endothelial progenitor cells to neovascularization (Review)[J]. Int J Mol Med, 2012,30(5):1000-1006.
[45]	Ambasta R K, Kohli H, Kumar P. Multiple therapeutic effect of endothelialprogenitor cell regulated by drugs in diabetes and diabetes related disorder[J].Journal of Translational Medicine, 2017, 15(1):185.
[46]	Akita T, Murohara T, Ikeda H, et al. Hypoxic Preconditioning Augments Efficacyof Human Endothelial Progenitor Cells for Therapeutic Neovascularization[J].Laboratory Investigation, 2003, 83(1):65-73.
[47]	Zhao B, Zhao Z, Sun X, et al. Effect of micro strain stress on proliferation ofendothelial progenitor cells inSvitro by the MAPK-ERK1/2 signaling pathway. [J].Biochemical Biophysical Research Communications, 2017, 492(2):206–211.

施引文献(7)

期刊类型引用(3)

1.	余少杰，张楠，李昌文，徐永，夏成雨. 血管祖细胞在烟雾病发病机制中的研究进展. 中国脑血管病杂志. 2021(02): 134-139 . 百度学术
2.	马小鹏，张华楸. 内皮祖细胞分泌的外泌体在颅脑损伤中作用的研究进展. 中国临床神经外科杂志. 2021(09): 724-726 . 百度学术
3.	刘冲，黄依雪，黄昆，袁杨棉，周依豪，郑凌. 组蛋白去甲基化酶UTX的研究进展. 中国细胞生物学学报. 2020(08): 1387-1394 . 百度学术