Abstract: Objective：To investigate the nursing care of diarrhea predominant irritable bowel syndrome (IBS-D) patients treated with fecal microbiota transplantation (FMT) and the improvement of diarrhea symptoms and comfort degree of patients after FMT. Methods：A retrospective analysis was conducted in IBS-D patients receiving nasointestinal FMT from April to October, 2016. We summarized the nursing interventions, recorded the adverse reactions after FMT, and used Bristol stool scale and general comfort questionnaire (GCQ) to estimate the changes of patients’ parameters. Results：Through the clinical treatment and a series of nursing interventions, the frequency of defecation and scores of Bristol fecal property decreased significantly (P<0.01), while the scores of GCQ had a significant improvement (P=0.004). However, some adverse reactions were observed, such as nausea, vomiting, fever, and an aggravation of diarrhea. Conclusions：FMT can improve patients’ diarrhea symptoms and improve their comfort, standardized nursing measures are worthy of clinical promotion.