Abstract: Objective：To construct plasmids of TDP-43 and its truncations to pcDNA3.1 vector with myc/his tag and explore the effect of myc/his-TDP-43 fusion protein on the alternative splicing of tau exon 10. Methods：The cDNA of TDP-43 and its truncations were amplified by polymerase chain reaction (PCR) and inserted into pcDNA3.1 after endonuclease cleavage. The recombinant plasmids were transfected into HEK-293FT cells and the expression of TDP-43 were detected by Western blots. TDP-43 or siTDP-43 was co transfected with tau mini gene, SI9/SI10, and the alternative splicing products of tau exon 10 was detected by RT-PCR. Results：pcDNA3.1/TDP-43·myc·his and the truncation plasmids were constructed successfully. Myc/his TDP-43 and truncation fusion proteins were expressed in HEK-293FT cells. Myc/his-TDP-43 fusion protein induced the expression of 4R-tau. Conclusions：Myc/his-TDP-43 fusion protein promoted 4R-tau expression. The truncation plasmids of TDP-43 might be helpful for the future study of the function of different domains of TDP-43 in tau pathology and alternative splicing of tau exon 10 in neurodegenerative diseases.