Abstract: Objective：To explore the two dimensional ultrasonography features of localized gallbladder adenomyomatosis. Methods：Thirty patients with pathologically proved localized gallbladder adenomyomatosis were enrolled in this study, and the two-dimensional ultrasound imaging characteristics were analyzed retrospectively. Results：Twenty-eight (93.1%) lesions located at the bottom of the gallbladder, 29(96.7%) lesions were broader base, 28 (93.1%) lesions were hypoechoic or iso-echoic, 29(96.7%) cases were with integrated gallbladder wall, cystic regions were detected in 21 (70.0%) lesions, tiny bright-echo were shown in 12 (40.0%) lesions. Color Doppler Flow imaging did not detect blood signal in 28 ( 93.1%) lesions. Diagnostic accuracy of localized gallbladder adenomyomatosis were 70% when combined with high-frequency ultrasound. Conclusions：The two-dimensional ultrasound imaging of localized gallbladder adenomyomatosis has certain characteristics. The combined application of high frequency ultrasound is helpful to improve the diagnostic coincidence rate.