Abstract: Objective：To explore the application value of three-dimensional visualization and virtual surgery system in liver surgery teaching. Methods：A total of 60 undergraduate interns from Fudan University School of Medicine, Grade 2012, were randomly divided into traditional teaching group (n=30) and three-dimension teaching group (n=30). The atlas of liver anatomy and liver surgery, liver model and operation video were used for teaching in traditional teaching group; the three-dimension reconstruction of liver model, virtual liver surgery system and operation video corresponding to real case were used for teaching in three-dimension teaching group. Standardized examination was given 6 class hours later and the questionnaire was used to investigate the students’ evaluation for the teaching methods. Results：The exam score of three-dimension teaching group (95.5±3.1) was significant higher than that of traditional teaching group (81.0±4.1, P<0.001). The excellence rate in three-dimension teaching group (93.3%) was also significant higher than that in traditional teaching group (40%, P<0.001). According to the questionnaire, the new teaching method was valued by all the students in three-dimension teaching group to be easy, vivid and interesting, which could arouse the learning enthusiasm, promote the mastery, improve the learning efficiency and strengthen the self-study ability. Conclusions：Novel clinical teaching using three-dimensional visualization and virtual surgery system is superior to traditional teaching in liver surgery teaching and is recommended for wide application.