Abstract: Objective：To preliminarily assess the stability, effectiveness, and safety of the electrophysiological stimulation feedback instrument DS21 for noninvasive screening diabetes. Methods：Totally 46 cases were selected and divided into health control group (NC,n=17),impaired glucose regulation group(IGR, n=15), and diabetes group (DM, n=14) based on OGTT and diabetic history. All cases signed informed consent, were performed routine checkup and biochemical examination, and collected history, and underwent three times tests by electrophysiological stimulation feedback instrument. Three conductance values of this instrument were recorded for each case, the deviation of average values were calculated, to evaluate its stability and effectiveness, and the adverse reactions were observed. Results：The maximum of the mean conductance value deviation was 4.4, the minimum value was 0.2 in the 46 subjects, which were all less than 5.0, The conductance values were 77.9±5.2，70.4±5.7, and 54.7±11.4 in NC, IGR, and DM group, respectively. The trend of conductance value was NC >IGR > DM group. The difference of conductance values among groups was statistically significant (P<0.01). After adjusting for gender，age, and body mass index (BMI), the difference of conductance values among groups was still statistically significant (P<0.01). There was no adverse event in the test in 46 cases. Conclusions：Electrophysiological stimulation feedback instrument DS21 is stable, reliable. The conductance values are different in subject with different glucose metabolism.