Abstract: Objective：To explore the detect in efficiency of Krebs von den Lungen-6 (KL-6) by latex agglutination method and evaluate its diagnostic value in patients with interstitial lung disease. Methods：The serum KL-6 levels of 44 healthy subjects were detected, and the reference interval provided by the kit manufacturer was verified. Serum KL-6 levels were compared between the ILD group, the other lung diseases group and the healthy subjects group. The diagnostic value of KL-6 for interstitial lung disease (ILD) was evaluated by drawing the ROC curve. Results：The serum KL-6 level of 43 in 44 healthy people (>90%) was within the reference range provided by the manufacturer. The median concentrations of KL-6 in the ILD group, the other lung diseases group and the healthy subjects group were 824 (187.9-8 214), 246 (101-2 129), and 168 (98-416) U/mL, respectively, and the differences were statistically significant (P<0.001). The sensitivity and specificity of KL-6 in diagnosis of ILD was 75% and 89.8%, respectively, with 500 U/mL as the cutoff value. The area under the ROC curve (AUC) of KL-6 for ILD diagnosis was 0.852 (95% CI 0.793-0.911, P<0.0001). Conclusions：The latex agglutination method for detecting serum KL-6 has good performance and KL-6 is a potential diagnostic index of ILD.