Abstract: Objective：To analyze the correlation between reexcision frequency of breastconserving surgery negative margins and clinical prognosis of patients with earlystage breast cancer. Methods：We collected the clinical information of early breast cancer patients treated with breast conserving therapy in the Third People's Hospital of Nantong City, Jiangsu Province, from January 2009 to October 2016. We analyzed the relationship between the frequency of re-excision and patient age, tumor size, lymph node status, hormone receptor status, Her-2, pathological type and prognosis of patients retrospectively. Results：In this study, 287 patients with early breast cancer who underwent breastconserving therapy were included. 191 (66.6%) patients acquired negative margins by one excision, 65 (22.6%) patients by twice excision, and 31 (10.8%) patients by thrice or more. Tumor size (P=0.010), pathological type (P< 0.001)， and HER-2 state (P=0.034) corrected with the frequency of re-excision. The median followup time was 5 years. Local recurrence rates were 6.3%, 9.2%, and 25.8% for one excision group, twice excision group, and thrice or more excision group, respectively and statistical difference was found among the three groups (P=0.002). No statistical difference was found among the three groups with regard to distant metastasis rates (P=0.989) and overall survival rates (P=0.326). Conclusions：Tumor size, type of pathology, HER-2 status were correlated with the frequency of re-excision required for negative margins in the breastconserving patients. The frequency of re-excision is positively correlated with local recurrence rates, but had no significant relationship with distant metastasis and overall survival rates.