Abstract: Objective：To investigate the expression of RNF38 in lung cancer, and its correlationship with clinicopathologic feature and prognosis. Methods：Tissue microarray was used to detect the level of RNF38 in 208 lung cancer patients, then its correlationship with clinicopathologic feature and prognosis was analyzed. Results：RNF38 expression was higher in lung cancer tissues compared with the corresponding adjacent normal lung tissues. High level of RNF38 was related significantly to tumor size (P=0.0002), tumor stage (P=0.011), lymph node metastasis (P=0.000 04) and histological type (P=0.030). High level of RNF38 indicates low overall survival rate. Single factor analysis showed that RNF38 expression was related to lung cancer patients’ prognosis. Conclusions：The high level of RNF38 could be a new marker in predicting the prognosis of lung cancer.