Abstract: Objective：To investigate the value of metoclopramide stimulation test in etiological diagnosis of hyperprolactinemia, and to analyze its possible influencing factors. Methods：Fiftythree patients with hyperprolactinemia treated in Department of Endocrinology of Huashan Hospital from November 2013 to March 2015 were enrolled. The metoclopramide stimulation test and the other related laboratory tests were performed to analyze the factors affecting test results. Results：In all the 53 hyperprolactinemia patients, 37 patients were diagnosed with prolactinoma. The diagnostic sensitivity of the metoclopramide stimulation test for hyperprolactinemia was 81.08%, the specificity was 68.75%, and the accuracy was 77.36%. The consistency test showed a Kappa value as 0.481 (P＜0.05). The result of metoclopramide stimulation test was independently associated with the prolactin level at 0 min (OR=1.035,95%CI 1.011-1.059,P＜0.001).Conclusions：The metoclopramide stimulation test has significant value in the etiological diagnosis of hyperprolactinemia. However, the result of metoclopramide stimulation test may be affected by the baseline of the prolactin level.