Abstract: Objective：To investigate the clinical significance of localized biopsy with body surface marker guided by mammography in the diagnosis and treatment of nonpalpable breast calcifications. Methods：The clinical data of 51 patients with nonpalpable breast lesions and calcification shown by mammography, grade 45 in BIRADS (breast imaging reporting and data system) from project collaboration units during January 2015 to May 2016 were retrospectively analyzed. Calcification excision biopsy guided by mammography was performed in all patients and further confirmed by pathological examination. Twentyfive patients’ biopsy were guided by stereotactic localizing wire, and 26 patients’ biopsy with body surface marker guided by mammography. Comparisons were made between the localization accuracy, size of negative resection margin and detection rate of malignant tumor. Results：In biopsy guided by stereotactic localizing wire, every patient underwent mammography 6 times on average, no complications occurred, and all patients had successful biopsy but one. The average negative resection margin was (30.6±8.7) mm, and the detection rate of malignant tumor was 11%. In biopsy with body surface marker guided by mammography, every patient underwent mammography 4 times on average, no complications occurred, and all patients had successful biopsy. The average negative resection margin was (29.1±5.6)mm, and the detection rate of malignant tumor was 20%. Conclusions：Under the cooperation of surgery and radiology, localized biopsy with body surface marker guided by mammography is high in accuracy, small in wound and low in cost, so it is more suitable for promotion in primary hospitals than biopsy guided by stereotactic localizing wire.