Abstract: Objective：To investigate the expression of PFKFB3 in human colon cancer and its clinical significance. Methods：Reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the mRNA and protein expression levels of PFKFB3 in cancer and adjacent tissues of 156 human colon cancer patients. The relationship of PFKFB3 expression with clinicopathological features and prognosis of colon cancer patients was analyzed by statistical analyses. Results：PFKFB3 mRNA and protein levels were significantly increased in tumor tissues compared to adjacent non-malignant tissues. Furthermore, correlation analysis indicated that PFKFB3 mRNA high expression in colon cancer tissues was significantly associated with intravascular cancer embolus, lymph node metastasis, and TNM staging（P<0.05）. Survival analysis showed a poor prognosis of patients with high expression of PFKFB3 mRNA level（P<0.05）. Conclusions：PFKFB3 is highly expressed in colon cancer. The expression level of PFKFB3 is correlated with the malignant phenotype and poor prognosis of colon cancer.