Abstract: Objective：To investigate the angiogenic effect and potential mechanism of sodium tanshinone ⅡA sulfonate (Tan ⅡA) in ischemic zebrafish model. Methods：Vascular endothelial growth factor receptor kinase inhibitor (VRKI) was used to induce vascular injury in zebrafish model. Then the ischemic zebrafish model was treated with 50, 100, 200 μmol/L Tan ⅡA, respectively. The effects of Tan ⅡA on ischemic zebrafish subintestinal veins (SIVs) and intersegmental vessels (ISVs) were observed. Meanwhile, the expression changes of three vascular endothelial growth factor (VEGF) signaling pathwayrelated genes (flt-1, flk-1A, flk-1B) were detected by realtime PCR. Results：In VRKIinduced vascular injury zebrafish model, Tan ⅡA gradually restored ISVs and SIVs in a concentrationdependent manner, reversed the downregulated expressions of three key VEGF signaling pathwayrelated genes (flt-1, flk-1A, flk-1B), and promoted angiogenesis. Conclusions：In the ischemic zebrafish model, Tan ⅡA plays a role in angiogenesis and vascular protection by regulating VEGF signaling pathway.