Abstract: Objective：To study the molecular mechanism of myocardial injury induced by traditional Chinese medicine Shengjiangsan. Methods：Male adult SD rats were randomly divided into normal group, model group, Shengjiangsan group and the p38 inhibitor group. By cecal ligation and puncture (CLP) of rat model of sepsis, the rat were observed at 4,8,12, and 24 h after operation. Shengjiangsan group and p38 inhibited rats respectively filled the stomach and subcutaneous injected the SB203580 in preoperative 2 h before giving Shengjiangsan. Blood samples from abdominal aortic were collected and heart tissue were detected. Observation and detection of each time node: mortality rate of rats, serum cTnI, BNP, IL-6 levels, myocardial p-p38MAPK protein, p-p38MAPK mRNA, IL-6 mRNA expression. Results：Model group has the 25% of mortality rates, while the Shengjiangsan group and the p38 inhibitor group both were 15%, which had no statistical significance. The cTnI and BNP were increased in model group, while the cTnI of 8 h, 12 h and 24 h in Shengjiangsan group were both improved (P<0.001), and the BNP of 4 h,12 h, and 24 h were all improved after BNP(P<0.05), but the difference between Shengjiangsan group and the p38 inhibitor group has no statistical significance in every time node. The IL-6 level in the model group has been higher and while in the Shengjiangsan group it has been decreased in every time node (P<0.001). The p-p38MARK protein level in model group grows higher, while the pp38MARK protein level in every time node of Shengjiangsan group has improved(P<0.05). The expression of p-p38MARK mRNA has all improved in the 8 h,12 h, and 24 h of Shengjiangsan group (P<0.05). The cardiac muscle Il-6 mRNA has improved in model group, while Shengjiangsan group and p38 inhibitor group of 4 h, 8 h, and 12 h has both improved (P<0.05), and the IL-6 mRNA become lower in the 12 h of Shengjiangsan group then of the p38 inhibitor group (P<0.05). Conclusions：Shengjiangsan can effectively reduce serum cTnI and BNP of the early sepsis rat. The molecular mechanism of that Shengjiangsan improved myocardial injury in sepsis may be related to the regulation of sepsis early rat by reduction of the myocardial p-p38MAPK protein level and the expression of p-p38MAPK mRNA and IL-6 mRNA, and also related to the inhibition the excessive inflammatory reaction.