Abstract: Objective：To investigate the location of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), and to analyze the relationship between the location and clinicopathologic feature and its prognostic significance in hepatocellular carcinoma (HCC). Methods：The location of hnRNP A1 was studied in an independent cohort of 323 HCC patients by immunohistochemistry, the hnRNP A1 location and clinicopathologic feature and its prognostic significance in HCC were concluded. Results：For hnRNP A1, there was weaktomoderate intensity immunoreactivity with nuclear expression in normal liver, while showed cytoplasmic and nuclear loaction in HCC. The cytoplasmic loaction of hnRNP A1 is significantly correlated with poor prognosis of HCC patients after operation. Conclusions：The hnRNP A1 is a great independent prognostic indicator, which can accurately predict the poor outcome of HCC patients after surgery.