Abstract: Objective：To observe expression changes of fibronectin (FN) and its effect on Gsn3 proliferation, migration and oligodendrocyte of spinal cord regeneration of Gekko japonicus, and also analyze the effects and possible mechanism of FN involved in spinal cord regeneration. Methods：Using degenerate PCR to obtain a special EST sequence of FN from Gekko japonicus tissue. The expression changes of FN mRNA in the regenerating spinal cord after tail amputation were detected by realtime quantitative polymerase chain reation (qRTPCR). In vitro, the human FN was used to culture Gekko oligodendrocyte cell line (Gsn3 cells). The morphology and proliferation of Gsn3 cells after FN treatment were also observed by DiI staining, whereas the migration was detected by Transwell. Results：During the Gekko spinal cord regeneration, FN mRNA expression is significantly increased at 1 dpa (1day post amputation) then decreased gradually with the time extension in distant spinal cord. FN is able to promote the proliferation and migration of Gsn3 cells, but could not promote the extension of process in vitro. Conclusions：FN participates in spinal cord regeneration by affecting oligodendrocyte proliferation and migration.