Construction and screening of effective sequences of lentiviral-mediated RNAi targeting PC4

Objective：To screen the effective sequences of lentivirus-mediated RNAi targeting PC4. Methods：Three candidate target sequences of PC4 RNAi were designed and cloned into lentivirus vectors, packaged into HEK-293T cell line, and then transfected the H9C2 cell line. The interference effects of target sequences were evaluated by real-time PCR and Western blotting analysis. Results：The lentiviral vector of RNAi containing the sequences targeting PC4 were successfully constructed, and effectively cloned into the H9C2 cell line. Real-time PCR and Western blotting results showed that PC4 expression in H9C2 cell line was downregulated significantly by shPC4-1. Conclusions：Sequences of lentivirus-mediated RNAi of PC4 are successfully screened.