Effects of GLI pathogenesis-related 1 gene on lung microbiome of mice exposed to cigarette smoke

Objective To explore the effect of GLI pathogenesis-related 1(GLIPR1) gene on the lung microbiome of mice exposed to cigarette smoke.

Methods Mice were exposed to cigarette smoke to established COPD mouse model.C57BL/6 mice and GLIPR1 konckout mice were randomly divided into 4 groups: wild-type control group (WT group), wild-type cigarette smoke exposure group (WT_SMOKE group), GLIPR1 gene knockout control group (GLIPR1 group), and GLIPR1 gene knockout cigarette smoke exposure group (GLIPR1_SMOKE group).Then 16S rRNA sequencing technology was used to analyze the differences among the lung microbiome of mice in 4 groups.

Results There were significant differences in β diversity among the lung microbiota of 4 groups (P_ANOSIM=0.003).At the genus level, there were 28 differential bacterial genera among the 4 groups.LEfSe analysis showed that compared with WT group, the proportion of Blautia in mice of WT_SMOKE group decreased by 0.25%(LDA score=3.137 log10, P=0.006).The proportion of Bifidobacterium(LDA score=3.465 log10, P=0.042) and Gemmatimonadetes.S0134_terrestrial_group(LDA score=3.115 log10, P=0.041) were highest in GLIPR1_SMOKE group, respectively.

Conclusions After exposure to cigarette smoke, the distribution of lung microbiome in GLIPR1 gene knockout mice is higher than that in WT mice, and Blautia, Bifidobacterium and Gemmatimonadetes.S0134_terrestrial_group play important roles.