Citation: | ZHU Wen-si, PENG Wen-jun, HAN Lin-xiao, et al. Effects of GLI pathogenesis-related 1 gene on lung microbiome of mice exposed to cigarette smoke[J]. Chin J Clin Med, 2023, 30(2): 238-244. DOI: 10.12025/j.issn.1008-6358.2023.20221340 |
To explore the effect of GLI pathogenesis-related 1(GLIPR1) gene on the lung microbiome of mice exposed to cigarette smoke.
Mice were exposed to cigarette smoke to established COPD mouse model.C57BL/6 mice and GLIPR1 konckout mice were randomly divided into 4 groups: wild-type control group (WT group), wild-type cigarette smoke exposure group (WT_SMOKE group), GLIPR1 gene knockout control group (GLIPR1 group), and GLIPR1 gene knockout cigarette smoke exposure group (GLIPR1_SMOKE group).Then 16S rRNA sequencing technology was used to analyze the differences among the lung microbiome of mice in 4 groups.
There were significant differences in β diversity among the lung microbiota of 4 groups (PANOSIM=0.003).At the genus level, there were 28 differential bacterial genera among the 4 groups.LEfSe analysis showed that compared with WT group, the proportion of Blautia in mice of WT_SMOKE group decreased by 0.25%(LDA score=3.137 log10, P=0.006).The proportion of Bifidobacterium(LDA score=3.465 log10, P=0.042) and Gemmatimonadetes.S0134_terrestrial_group(LDA score=3.115 log10, P=0.041) were highest in GLIPR1_SMOKE group, respectively.
After exposure to cigarette smoke, the distribution of lung microbiome in GLIPR1 gene knockout mice is higher than that in WT mice, and Blautia, Bifidobacterium and Gemmatimonadetes.S0134_terrestrial_group play important roles.
[1] |
CHRISTENSON S A, SMITH B M, BAFADHEL M, et al. Chronic obstructive pulmonary disease[J]. Lancet, 2022, 399(10342): 2227-2242. DOI: 10.1016/S0140-6736(22)00470-6
|
[2] |
WHO. Global Health Estimates: Life expectancy and leading causes of death and disability[EB/OL]. (2020-12-09)[2022-6-03]. https://www.who.int/data/gho/data/themes/mortality-and-global-health-estimates.
|
[3] |
JONES B, DONOVAN C, LIU G, et al. Animal models of COPD: what do they tell us?[J]. Respirology, 2017, 22(1): 21-32. DOI: 10.1111/resp.12908
|
[4] |
DICKSON R P, ERB-DOWNWARD J R, MARTINEZ F J, et al. The microbiome and the respiratory tract[J]. Annu Rev Physiol, 2016, 78: 481-504. DOI: 10.1146/annurev-physiol-021115-105238
|
[5] |
CUTHBERTSON L, WALKER A W, OLIVER A E, et al. Lung function and microbiota diversity in cystic fibrosis[J]. Microbiome, 2020, 8(1): 45. DOI: 10.1186/s40168-020-00810-3
|
[6] |
ROGERS G B, ZAIN N M, BRUCE K D, et al. A novel microbiota stratification system predicts future exacerbations in bronchiectasis[J]. Ann Am Thorac Soc, 2014, 11(4): 496-503. DOI: 10.1513/AnnalsATS.201310-335OC
|
[7] |
ZHANG Q L, COX M, LIANG Z K, et al. Airway microbiota in severe asthma and relationship to asthma severity and phenotypes[J]. PLoS One, 2016, 11(4): e0152724. DOI: 10.1371/journal.pone.0152724
|
[8] |
HALDAR K, GEORGE L, WANG Z, et al. The sputum microbiome is distinct between COPD and health, independent of smoking history[J]. Respir Res, 2020, 21(1): 183. DOI: 10.1186/s12931-020-01448-3
|
[9] |
MURPHY E V, ZHANG Y, ZHU W, et al. The human glioma pathogenesis-related protein is structurally related to plant pathogenesis-related proteins and its gene is expressed specifically in brain tumors[J]. Gene, 1995, 159(1): 131-135. DOI: 10.1016/0378-1119(95)00061-A
|
[10] |
PENG W J, WU Y Y, ZHANG G, et al. GLIPR1 protects against cigarette smoke-induced airway inflammation via PLAU/EGFR signaling[J]. Int J Chron Obstruct Pulmon Dis, 2021, 16: 2817-2832. DOI: 10.2147/COPD.S328313
|
[11] |
HUANG Y J, NARIYA S, HARRIS J M, et al. The airway microbiome in patients with severe asthma: associations with disease features and severity[J]. J Allergy Clin Immunol, 2015, 136(4): 874-884. DOI: 10.1016/j.jaci.2015.05.044
|
[12] |
SVERRILD A, KⅡLERICH P, BREJNROD A, et al. Eosinophilic airway inflammation in asthmatic patients is associated with an altered airway microbiome[J]. J Allergy Clin Immunol, 2017, 140(2): 407-417.e11. DOI: 10.1016/j.jaci.2016.10.046
|
[13] |
SZE M A, DIMITRIU P A, HAYASHI S, et al. The lung tissue microbiome in chronic obstructive pulmonary disease[J]. Am J Respir Crit Care Med, 2012, 185(10): 1073-1080. DOI: 10.1164/rccm.201111-2075OC
|
[14] |
RASHIDI A, PELED J U, EBADI M, et al. Protective Effect of Intestinal Blautia Against Neutropenic Fever in Allogeneic Transplant Recipients[J]. Clin Infect Dis, 2022, 75(11): 1912-1920. DOI: 10.1093/cid/ciac299
|
[15] |
KIM S G, BECATTINI S, MOODY T U, et al. Microbiota-derived lantibiotic restores resistance against vancomycin-resistant Enterococcus[J]. Nature, 2019, 572(7771): 665-669. DOI: 10.1038/s41586-019-1501-z
|
[16] |
CUI S M, GU J Y, LIU X M, et al. Lactulose significantly increased the relative abundance of Bifidobacterium and Blautia in mice feces as revealed by 16S rRNA amplicon sequencing[J]. J Sci Food Agric, 2021, 101(13): 5721-5729. DOI: 10.1002/jsfa.11181
|
[17] |
VERSTRAETEN S, SENCIO V, RAISE A, et al. Description of a newly isolated Blautia faecis strain and its benefit in mouse models of post-influenza secondary enteric and pulmonary infections[J]. Nutrients, 2022, 14(7): 1478. DOI: 10.3390/nu14071478
|
[18] |
KAWASE M, HE F, KUBOTA A, et al. Oral administration of lactobacilli from human intestinal tract protects mice against influenza virus infection[J]. Lett Appl Microbiol, 2010, 51(1): 6-10.
|
[19] |
EZEJI J C, SARIKONDA D K, HOPPERTON A, et al. Parabacteroides distasonis: intriguing aerotolerant gut anaerobe with emerging antimicrobial resistance and pathogenic and probiotic roles in human health[J]. Gut Microbes, 2021, 13(1): 1922241. DOI: 10.1080/19490976.2021.1922241
|
[20] |
VIEIRA A T, ROCHA V M, TAVARES L, et al. Control of Klebsiella pneumoniae pulmonary infection and immunomodulation by oral treatment with the commensal probiotic Bifidobacterium longum 5(1A)[J]. Microbes Infect, 2016, 18(3): 180-189. DOI: 10.1016/j.micinf.2015.10.008
|
[21] |
BUDDEN K F, GELLATLY S L, WOOD D L, et al. Emerging pathogenic links between microbiota and the gut-lung axis[J]. Nat Rev Microbiol, 2017, 15(1): 55-63. DOI: 10.1038/nrmicro.2016.142
|