| Citation: |
ZHU Wen-si, PENG Wen-jun, HAN Lin-xiao, et al. Effects of GLI pathogenesis-related 1 gene on lung microbiome of mice exposed to cigarette smoke[J]. Chin J Clin Med, 2023, 30(2): 238-244. DOI: 10.12025/j.issn.1008-6358.2023.20221340
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To explore the effect of GLI pathogenesis-related 1(GLIPR1) gene on the lung microbiome of mice exposed to cigarette smoke.
Mice were exposed to cigarette smoke to established COPD mouse model.C57BL/6 mice and GLIPR1 konckout mice were randomly divided into 4 groups: wild-type control group (WT group), wild-type cigarette smoke exposure group (WT_SMOKE group), GLIPR1 gene knockout control group (GLIPR1 group), and GLIPR1 gene knockout cigarette smoke exposure group (GLIPR1_SMOKE group).Then 16S rRNA sequencing technology was used to analyze the differences among the lung microbiome of mice in 4 groups.
There were significant differences in β diversity among the lung microbiota of 4 groups (PANOSIM=0.003).At the genus level, there were 28 differential bacterial genera among the 4 groups.LEfSe analysis showed that compared with WT group, the proportion of Blautia in mice of WT_SMOKE group decreased by 0.25%(LDA score=3.137 log10, P=0.006).The proportion of Bifidobacterium(LDA score=3.465 log10, P=0.042) and Gemmatimonadetes.S0134_terrestrial_group(LDA score=3.115 log10, P=0.041) were highest in GLIPR1_SMOKE group, respectively.
After exposure to cigarette smoke, the distribution of lung microbiome in GLIPR1 gene knockout mice is higher than that in WT mice, and Blautia, Bifidobacterium and Gemmatimonadetes.S0134_terrestrial_group play important roles.
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