The long non-coding RNA LINC00922 promotes the proliferation, migration and invasion of pancreatic ductal adenocarcinoma cell line

Objective To explore the expression of long non-coding RNA LINC00922 in pancreatic ductal adenocarcinoma (PDAC) tissues and PDAC cell lines and its function on proliferation and invasion of PDAC cell lines.

Methods Appling the illumina HiSeq X Ten to high-throughput sequence four pairs of PDAC tissues and corresponding adjacent normal tissues during 2015-2016.Establishing the lncRNA and mRNA expression profile with the sequencing results.LINC00922 was highly expressed in PDAC tissues and was selected for further study.The expression of LINC00922 was verified by the RT-qPCR with another 10 pairs of pancreatic tissues and adjacent normal tissues.The function of LINC00922 on proliferation and invasion of pancreatic cancer cell lines was explored by CCK-8 cell proliferation assay, wound-healing assay and Transwell assay.Paired samples were analyzed by paired sample t test, or independent sample t test.

Results Through high-throughput sequencing, 470 differentially expressed lncRNA and 4 373 differentially expressed mRNA were discovered in PDAC tissues.The lncRNA LINC00922 was over-expressed in PDAC.With another 10 paired pancreatic cancer tissues and adjacent normal tissues, the expression of LINC00922 was over-expressed in PDAC tissues (P < 0.05).CCK-8 cell proliferation assay showed that the proliferation activity of BxPC-3 was suppressed by down-expressing of LINC00922(P < 0.05).The wound healing assay showed that pancreatic cancer cells in LINC00922 knocking-down group was significantly more slowly closing to the scratch area than the negative control group (P < 0.05).The transwell assay showed that the number of migrating cells in LINC00922 knocking-down group was significantly lower than the negative control group (P < 0.05).

Conclusions LINC00922 is over-expressed in PDAC and promotes the proliferation, migration and invasion of PDAC cells.