Objective To assess the expression level of Ras association and DIL domains (Radil), and its effect on hepatocellular carcinoma (HCC).
Methods The expression of Radil was measured by immunohistochemistry and Western blot in HCC tissue samples. Then the expression of Radil was measured in several HCC cell lines by Western blot, and Rnock-down and over-expression were carried out in HCC cell lines respectively to test cell proliferation and movement ability.
Results The expression of Radil in HCC tissues was significantly higher than that in corresponding adjacent tissues. The expression of Radil promoted HCC cell proliferation and migration ability, and the apoptosis-related protein expression levels were decreased.
Conclusions This study suggests that Radil is overexpressed in HCC. It may play an important role in carcinogenesis or aggression and may be a useful marker for the prognosis evaluation of HCC.
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