Objective To explore the diagnostic value of fine needle aspiration cytology (FNAC) combined with fluorescence quantitative polymerase chain reaction (qPCR) for suspected extrapulmonary tuberculosis.
Methods A total of 57 patients probably infected by mycobacterium tuberculosis (TB) or needed to be excluded from infected TB were selected, and their puncture fluid of body surface cysts were collected. DNA was extracted from the puncture fluid and the qPCR method was used to detect the DNA of TB. The results of qPCR detection, FNAC, and clinical diagnosis were compared.
Results Based on clinical diagnosis (N=23), the diagnostic coincidence rate of FNAC was 82.61% (19/23), and the coincidence rate of qPCR test was 86.96% (20/23). The diagnostic coincidence of the combination of two methods was 92.98% (22/23).
Conclusions Fine needle aspiration cytology combined with qPCR can effectively diagnose the extrapulmonary tuberculosis, and the diagnosis rate is higher than single method.
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