Objective To investigate the results of automated microcolumn gel method in direct antiglobulin test screening and the clinical characteristics of false positive results.
Methods A total of 1205 samples from Dec. 2016 to Nov. 2017 were included in the study. Automated microcolumn gel method was used for direct antiglobulin test screening, when the result was positive, manual tube method was used to further define the type of IgG/C3d.
Results Out of the 1 205 samples, 180 (14.9%) were positive, which were graded as follows:𠦌, 6 cases; ⫵, 23 cases; ⫲, 41 cases; +, 95 cases; +/-, 15 cases. And results of 18 cases were positive using the gel method (+, 13; +/-, 5), and were negative using the tube method and mostly were from patients with lymphatic hematopoietic system disease or autoimmune disease.
Conclusions Automated microcolumn gel method has the advantages of standardized operation, clear and preservable results and high sensitivity, but may have a certain proportion of false positives which can be excluded by strict pretreatment quality control.
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