| RNF157 participates in the ERK pathway activation to promote melanoma cells proliferation by down-regulating the expression of caveolin-1 |
| Received:March 28, 2023 Revised:April 14, 2023 Click here to download the full text |
| Citation of this paper:DENG Xin-yi,GAO Zi-xu,WANG Lu,SHEN Kang-jie,REN Ming,YANG Yang,LI Jian-rui,ZHANG Si-min,YANG Yan-wen,ZHANG Yong,WEI Chuan-yuan,GU Jian-ying.RNF157 participates in the ERK pathway activation to promote melanoma cells proliferation by down-regulating the expression of caveolin-1[J].Chinese Journal of Clinical Medicine,2023,30(2):257-264 |
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| Author Name | Affiliation | E-mail | | DENG Xin-yi | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | GAO Zi-xu | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | WANG Lu | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | SHEN Kang-jie | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | REN Ming | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | YANG Yang | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | LI Jian-rui | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | ZHANG Si-min | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | YANG Yan-wen | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | ZHANG Yong | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | WEI Chuan-yuan | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | wei.chuanyuan@zs-hospital.sh.cn | | GU Jian-ying | Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | prof_jianyinggu@163.com |
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| Abstract:Objective To explore the role and mechanism of RING finger protein 157 (RNF157) in melanoma cells proliferation. Methods The RNF157 overexpression melanoma cell line was constructed by lentivirus transfection technique, and the RNF157 knockout melanoma cell line was constructed by small interfering RNA (siRNA). The transfection effect was verified by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) and Western blotting. MTT assay and clone formation assay were used to analyze the cell proliferation ability of RNF157 overexpression melanoma cells and knockdown RNF157 melanoma cells. The proliferation ability of RNF157 overexpressing melanoma cells in vitro was detected by subcutaneous tumorigenesis test and immunohistochemical staining in nude mice. The potential mechanism of RNF157 in ERK pathway was explored by co-immunoprecipitation (Co-IP) combined with mass spectrometry, proteomics and ubiquitinomics. Results Immunohistochemical staining showed that the expression of RNF157 in melanoma tissue was higher than that in adjacent normal tissue (P＜0.01). The results of qRT-PCR and Western blotting showed that the expression level of RNF157 in overexpression group was significantly higher than that in control group (P＜0.000 1), the transfection efficiency of si3 was the highest, and the expression level of RNF157 in si3 group was significantly lower than that in control group (P＜0.01). The results of MTT assay and colony formation assay showed that overexpression of RNF157 could promote the proliferation of melanoma cells, and knockdown RNF157 could inhibit the proliferation of melanoma cells. The results of Western blotting showed that overexpression of RNF157 could activate ERK pathway in melanoma cells. Co-IP combined with mass spectrometry, proteomics and ubiquitinomics analysis showed that RNF157 could bind to caveolin-1 (CAV1) and down-regulate its expression level, thus regulating the ERK pathway. Conclusions RNF157 can promote the proliferation of melanoma cells in vitro and in vivo. In melanoma cells, RNF157 participates in the activation of ERK pathway by binding and down-regulating the expression of CAV1. |
| keywords:RNF157 melanoma cell proliferation ERK pathway |
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