| Effect of astragaloside Ⅳ alleviating senescence of adipose-derived stem cells under high-glucose environment |
| Received:February 14, 2023 Revised:April 06, 2023 Click here to download the full text |
| Citation of this paper:GAO Jun-li,XU Jing,YU Chun-li,LIU Kun,WANG Wei-wei,XU Guo-xiong.Effect of astragaloside Ⅳ alleviating senescence of adipose-derived stem cells under high-glucose environment[J].Chinese Journal of Clinical Medicine,2023,30(3):460-467 |
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| Author Name | Affiliation | E-mail | | GAO Jun-li | Department of Nephrology, Jinshan Branch, Shanghai Sixth People's Hospital, Shanghai 201500, China | | | XU Jing | Department of Nephrology, Jinshan Branch, Shanghai Sixth People's Hospital, Shanghai 201500, China | | | YU Chun-li | Department of Nephrology, Jinshan Branch, Shanghai Sixth People's Hospital, Shanghai 201500, China | | | LIU Kun | Department of Nephrology, Jinshan Branch, Shanghai Sixth People's Hospital, Shanghai 201500, China | 13701733598@163.com | | WANG Wei-wei | Department of Nephrology, Navy Characteristic Medical Center, Shanghai 2012033, China | w.vwei@163.com | | XU Guo-xiong | Clinical Medical Research Center, Jinshan Hospital, Fudan University, Shanghai 200032, China | |
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| Abstract:Objective To investigate the effect of astragaloside Ⅳ (Ast) alleviating human adipose-derived stem cells (hADSCs) senescence induced by high glucose. Methods The high glucose concentration was screened by culturing hADSCs with glucose at different concentrations. The optimal Ast concentration was screened by culturing hADSCs with Ast at different concentrations. hADSCs were divided into four groups:the control group was treated with medium containing 5.5 mmol/L glucose; the mannitol group was treated with medium containing 5.5 mmol/L glucose and 19.5 mmol/L mannitol; the high glucose group was treated with medium containing glucose at optimal high-concentration; and the Ast group was treated with medium containing Ast at optimal concentration and glucose at optimal high-concentration. After 72 hours of culture, SA-β-Gal staining was used to observe cell senescence; Annexin Ⅴ-FITC/PI was used to detect apoptosis; qRT-PCR and Western blotting were used to detect the expressions of p16, Pink1, Parkin, LC3 (LC3Ⅱ/Ⅰ), and p62 in hADSCs; and transmission electron microscopy was used to observe mitochondria and autophagosomes. Results The optimal high glucose concentration was 25 mmol/L and the optimal Ast concentration was 20 mg/L. Annexin Ⅴ-FITC/PI showed that the apoptosis of hADSCs in the high glucose group was increased compared with the control group (P<0.001), and the apoptosis of hADSCs in the Ast group was decreased compared with the high glucose group (P<0.001). SA-β-Gal staining showed that the senescent cells increased in the high glucose group compared with the control group (P<0.001), and the senescent cells decreased in the Ast group compared with the high glucose group (P<0.001). qRT-PCR and Western blotting showed that compared with the control group, the expression levels of p16 and p62 in hADSCs in the high glucose group were higher (P<0.001), and the expression levels of LC3, Pink1, and Parkin were lower (P<0.001); compared with the high glucose group, the expression levels of p16 and p62 in hADSCs in Ast group were lower (P<0.001), and the expression levels of LC3, Pink1, and Parkin were higher (P<0.01). Transmission electron microscopy showed that the mitochondria in the high glucose group were swollen and deformed, with a large volume and a small number of autophagosomes; after Ast intervention, a small number of swollen and deformed mitochondria were observed, with a large number of autophagosomes. Conclusions Ast could alleviate hADSCs senescence induced by high glucose, and the mechanism may be related to the up-regulation of mitophagy. |
| keywords:human adipose-derived stem cells astragaloside Ⅳ high glucose senescence autophagosome |
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