| Down-regulation of integrin α5β1 participates in the formation of acute type A aortic dissection in human |
| Received:October 20, 2022 Revised:January 18, 2023 Click here to download the full text |
| Citation of this paper:XUE Ming-ming,YANG Yi-lin,XING Ling-yu,XU Fei-xiang,WANG Sheng,CHEN Yu-mei,CHEN Bin,SONG Zhen-ju,YAO Chen-ling,GU Guo-rong,TONG Chao-yang.Down-regulation of integrin α5β1 participates in the formation of acute type A aortic dissection in human[J].Chinese Journal of Clinical Medicine,2023,30(2):265-271 |
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| Author Name | Affiliation | E-mail | | XUE Ming-ming | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | YANG Yi-lin | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | XING Ling-yu | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | XU Fei-xiang | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | WANG Sheng | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | CHEN Yu-mei | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | CHEN Bin | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | SONG Zhen-ju | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | YAO Chen-ling | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | GU Guo-rong | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | TONG Chao-yang | Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China | tong.chaoyang@zs-hospital.sh.cn |
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| Abstract:Objective To explore expression levels of integrin α5β1 in lesion tissues of patients with acute type A aortic dissection (AAAD) and human aortic smooth muscle cells (HASMC) and the possible regulatory mechanism. Methods Immunohistochemistry was used to detect expression levels of integrin α5β1, phosphorylated focal adhesion kinase (p-FAK) and Caspase 3 proteins in the lesion tissues of AAAD patients. Immunofluorescence was used to detect integrins α5β1 and p-FAK expressions in HASMC. siRNA was used to downregulate integrin α5β1 in HASMC, CCK-8 and flow cytometry were used to detect the proliferation activity and apoptosis of HASMC, respectively. Results The expression of integrin α5β1 at lesion tissues in AAAD patients was decreased (P＜0.05). Integrins α5β1 and FAK expressed in the same sites of normal HASMC. After down regulating expression of integrin α5β1 in HASMC, the smooth muscle cells showed decreased proliferation and increased apoptosis (P＜0.001). The expression of α5β1 and FAK proteins was downregulated (P＜0.001). Conclusions Integrin α5β1/FAK signal pathway in the aorta of AAAD patients is down regulated, and the HASMC proliferation decreases and apoptosis increases after this signal pathway is inhibited. |
| keywords:integrin α5β1 acute type A aortic dissection aortic smooth muscle cell |
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