| Dynamic changes of inflammatory factors and T cell subsets in different tissues in septic mice |
| Received:June 30, 2022 Revised:August 11, 2022 Click here to download the full text |
| Citation of this paper:SUN Qi,DING Chun-lei,FAN Qian,PAN Zhi-de,TAN Jing,QIAN Yi-ming,ZHAO Lei,QIAN Feng-hua.Dynamic changes of inflammatory factors and T cell subsets in different tissues in septic mice[J].Chinese Journal of Clinical Medicine,2022,29(6):999-1005 |
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| Author Name | Affiliation | E-mail | | SUN Qi | Department of Emergency, Yueyang Hospital of Integrated Traditonal Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China | | | DING Chun-lei | Department of Emergency, Yueyang Hospital of Integrated Traditonal Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China | | | FAN Qian | Department of Emergency, Yueyang Hospital of Integrated Traditonal Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China | | | PAN Zhi-de | Department of Emergency, Yueyang Hospital of Integrated Traditonal Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China | | | TAN Jing | Department of Emergency, Yueyang Hospital of Integrated Traditonal Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China | | | QIAN Yi-ming | Department of Emergency, Yueyang Hospital of Integrated Traditonal Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China | | | ZHAO Lei | Department of Emergency, Yueyang Hospital of Integrated Traditonal Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China | | | QIAN Feng-hua | Department of Emergency, Yueyang Hospital of Integrated Traditonal Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China | smileqian1975@qq.com |
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| Abstract:Objective To investigate the changes of inflammatory infiltration degree and immune status of different tissues in sepsis mice established by cecal ligation and perforation (CLP) during the development of the disease. Methods 40 mice were used to establh, 48h and 72h after operation. The percentages of Treg and Th17 in spleen and the proportions of CD4＋T and CD8＋T lymphocytes in lamina propria of ileum mucosa were detected by flow cytometry.40 mice underwent sham operation were as the control. Results The IL-6 level in lung and ileum reached the peak at 6h after operation, and the IL-6 level in plasma reached the peak at 12h after CLP, which was significantly higher than that in the sham group at the same time point (P＜0.01).The IL-10 level in ileum reached the peak at 6h after CLP, which was significantly higher than that in sham group (P＜0.01).The level of IL-2 in ileum tissue increased continuously after CLP, and reached the peak at 24 h, which was significantly higher than that in sham group (P＜0.01).The percentages of Th17 and Treg in spleen at 6h and 24h after CLP were significantly higher than those in sham group at the same time point (P＜0.01); the CD4＋T cell percentage in lamina propria of ileum mucosa reached the highest at 12h after CLP, and CD8＋T cell percentage reached the highest at 24h after CLP, which were significantly higher than those in sham group (P＜0.01).The percentages of Th17, Treg in spleen, and CD4＋T and CD8＋T cells in lamina propria of ileum mucosa all decreased at 24h after operation in the two groups. Conclusions The infiltration degrees of inflammatory factors are different in different tissues during sepsis, and the inflammatory reaction in intestinal mucosa appears earlier and last longer; during the middle and late stage of sepsis, CD8＋T cells decreasing and the Treg ratio relativly increasing imply that lymphocyte proliferation is decreased. |
| keywords:sepsis T lymphocyte regulatory T cell ileum intestinal mucosa inflammatory cytokine |
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