| Associations of c-Met, Fascin and CD44 with clinical pathological features and prognosis of patients with hepatocellular carcinoma |
| Received:April 11, 2022 Revised:September 12, 2022 Click here to download the full text |
| Citation of this paper:ZHU Lin,CHEN Li,XIAO Feng,ZHANG Hai-yan,GU Chun-yan.Associations of c-Met, Fascin and CD44 with clinical pathological features and prognosis of patients with hepatocellular carcinoma[J].Chinese Journal of Clinical Medicine,2022,29(5):745-752 |
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| Author Name | Affiliation | E-mail | | ZHU Lin | Department of Pathology, Nantong 3rd People's Hospital, Nantong Third Hospital Affiliated Nantong University, Nantong 226000, Jiangsu, China | | | CHEN Li | Department of Pathology, Nantong University Medical School, Nantong 226001, Jiangsu, China | | | XIAO Feng | Department of Pathology, Nantong 3rd People's Hospital, Nantong Third Hospital Affiliated Nantong University, Nantong 226000, Jiangsu, China | | | ZHANG Hai-yan | Department of Pathology, Nantong 3rd People's Hospital, Nantong Third Hospital Affiliated Nantong University, Nantong 226000, Jiangsu, China | | | GU Chun-yan | Department of Pathology, Nantong 3rd People's Hospital, Nantong Third Hospital Affiliated Nantong University, Nantong 226000, Jiangsu, China | guchunyan143@aliyun.com |
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| Abstract:Objective To explore the association of c-Met, Fascin and CD44 with the clinical pathological fetures and prognosis of patients with hepatocellular carcinoma (HCC). Methods The RNA SEqV2 data of c-Met, Fascin and CD44 in HCC from the cancer genome atlas (TCGA) were downloaded (n=50). The mRNA levels of c-Met, Fascin and CD44 between cancer and paracancer tissues were compared. Western blotting was used to detect the expression levels of c-Met, Fascin and CD44 proteins in 6 cases of cancer and the matched paracancer liver tissues. The expression levels of c-Met, Fascin and CD44 proteins were examined in cancer and paracancer liver tissues from 186 patients by immunohistochemistry (IHC). The associations of c-Met, Fascin and CD44 with clinical pathological factors and prognosis of patients were analyzed. Results The data from TCGA showed that the mRNA levels of c-Met, Fascin and CD44 were significantly higher in cancer tissues than those in the paracancer tissues (P<0.01). Western blotting results showed that the protein levels of c-Met, Fascin and CD44 in fresh cancer tissues were significantly higher than those in the paracancer tissues (P<0. 05). IHC results showed the c-Met expressed in 60.2% (112/186) of cancer tissues and 32.8% (61/186) of paracancer tissues, Fascin expressed in 56.5% (105/186) of cancer tissues and 24.7% (46/186) of paracancer tissues, and CD44 expressed in 73.1% (136/186) of cancer tissues and 50.5% (94/186) of paracancer tissues. The protein levels of c-Met, Fascin and CD44 in cancer tissues were higher than those in paracancer tissues (P<0.01). The expressions of c-Met,Fascin and CD44 proteins in patients with vascular invasion or poor differentiation tumor and in recurrence patients were higher (P<0.05). Spearman analysis showed that there was a positive correlation between c-Met and Fascin (r=0.349 5, P<0.001). Kaplan-Meier and multivariate Cox analysises all showed that higher levels of c-Met, Fascin and CD44 were risk factors for HCC patients survival (P<0.05). Conclusion c-Met, Fascin and CD44 expressions are increased in HCC cancer tissues, are higher in vascular invasion, lower tumor differentiation, and recurrence patients, and may be independent risk factors for HCC prognosis. |
| keywords:c-Met Fascin CD44 hepatocellular carcinoma vascular invasion relapse |
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