| Preparation and ultrasonic imaging ability of CD147-targeted nanobubbles |
| Received:May 26, 2021 Revised:September 15, 2021 Click here to download the full text |
| Citation of this paper:YUAN Hai-xia,GUAN Pei-shan,JIN Yun-jie,YANG Ping,WU Ai-qin,WANG Wen-ping.Preparation and ultrasonic imaging ability of CD147-targeted nanobubbles[J].Chinese Journal of Clinical Medicine,2022,29(1):84-87 |
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| Author Name | Affiliation | E-mail | | YUAN Hai-xia | Department of Ultrasound, Zhongshan Hospital, Fudan University (Xiamen Branch), Xiamen 361015, Fujian, China
Department of Ultrasound, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | GUAN Pei-shan | Department of Ultrasound, Zhongshan Hospital, Fudan University (Xiamen Branch), Xiamen 361015, Fujian, China | | | JIN Yun-jie | Department of Ultrasound, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Institute of Ultrasound Medicine and Engineering, Fudan University, Shanghai 200032, China | | | YANG Ping | Department of Ultrasound, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | WU Ai-qin | Department of Ultrasound, Zhongshan Hospital, Fudan University (Xiamen Branch), Xiamen 361015, Fujian, China | | | WANG Wen-ping | Department of Ultrasound, Zhongshan Hospital, Fudan University (Xiamen Branch), Xiamen 361015, Fujian, China
Department of Ultrasound, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Institute of Ultrasound Medicine and Engineering, Fudan University, Shanghai 200032, China | puguang61@126.com |
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| Abstract:Objective To prepare CD147-targeted nanobubble contrast agent and to evaluate its physicochemical properties, liver cancer cell-targeting ability, and ultrasound imaging ability. Methods PLGA-PEG, DSPE-PEG2000-COOH, and perfluoropropane saturated aqueous solution were mixed and combined with CD147 antibody by coupling reaction to prepare CD147-targeted nanobubbles. CD147-targeted and non-targeted nanobubbles were co-incubated with MHCC-97H cells, respectively, and the targeting ability of nanobubbles was evaluated. The particle size of CD147-targeted microbubbles was detected at 1 d and 3 d after the preparation, and the ultrasonic imaging was performed in vitro and in nude mice. Results CD147-targeted nanobubbles were uniformly distributed in the solution, and immunofluorescence results showed that CD147-targeted nanobubbles bond to MHCC-97H cells significantly more than the non-targeted nanobubbles (P<0.01). The average diameter of targeted bubbles was (188.1±4.8) nm and (193.1±11.3) nm at 1 d and 3 d after preparation, respectively. At the 1 d and 3 d, the targeted microbubbles had good ultrasound imaging ability both in vitro and in vivo. Conclusion The prepared CD147-targeted nanobubbles have stable physical and chemical properties, can specifically bind to liver cancer cells, and have good ultrasound imaging ability in vitro and in vivo. They can be used as a nano-scale molecular probe for ultrasonic molecular imaging and targeted therapy of liver cancer. |
| keywords:CD147 hepatoma cell ultrasonic molecular imaging target-seeking ability |
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