| Effects of cAMP-response element binding protein 1 silencing on biological behaviors of breast cancer cells |
| Received:April 16, 2021 Revised:June 14, 2021 Click here to download the full text |
| Citation of this paper:XIN Zhao-chen,WU Xi,BANG SOYEON,WANG Hong,YANG Zi-ang,ZHANG Hong-wei.Effects of cAMP-response element binding protein 1 silencing on biological behaviors of breast cancer cells[J].Chinese Journal of Clinical Medicine,2022,29(2):153-160 |
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| Author Name | Affiliation | E-mail | | XIN Zhao-chen | Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | WU Xi | Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | BANG SOYEON | Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | WANG Hong | Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | YANG Zi-ang | Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | ZHANG Hong-wei | Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | zhang.hongwei@zs-hospital.sh.cn |
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| Abstract:Objective To explore the effects of cAMP-response element binding protein 1 (CREB1) gene silencing on the proliferation, apoptosis, migration, and invasion of breast cancer MCF-7 and MDA-MB-231 cell lines. Methods Two short hairpin RNAs (shRNAs) were designed and constructed for CREB1 gene sequence, and then transfected into human breast cancer MCF-7 and MDA-MB-231 cell lines to silence CREB1 expression. The experimental groups included shCREB1#1 and shCREB1#2 groups, and the shSCR empty plasmid was transfected into the above cell lines as the negative control group. The transfection efficiency was determined by real-time quantitative PCR and Western blotting methods. CCK-8 assay was used to detect cell proliferation. Colony formation experiment was used to detect the colony formation ability of breast cancer cells. Flow cytometry was used to detect the cell cycle and apoptosis rate. Cell wound assay and transwell assay were used to detect cell migration and invasion, respectively. The expression of cell cycle and apoptosis-related proteins were detected by Western blotting. Results In MCF-7 and MDA-MB-231 cell lines, compared with the negative control group, the relative CREB1 mRNA and protein expression levels were decreased in shCREB1#1 and shCREB1#2 groups (P＜0.001). After CREB1 silencing, the proliferation, colony formation, migration, and invasion abilities of MCF-7 and MDA-MB-231 cell lines were decreased. Meanwhile, the cell apoptosis rate was increased (P＜0.05). CREB1 silencing could down-regulate the expression levels of cell cycle-related proteins (CDK2, CDK4, CDK6, and Cyclin D1) and anti-apoptotic proteins (Bcl-2 and Survivin) and up-regulate the expression of pro-apoptotic proteins (Caspase 3 and Bax, P＜0.05). Conclusion CREB1 silencing could inhibit the proliferation, migration, and invasion of breast cancer cells and induce cell apoptosis. |
| keywords:cAMP-response element binding protein 1 breast cancer proliferation apoptosis migration MCF-7 MDA-MB-231 |
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