| Proteomics study of differentially expressed proteins in patients with different stages of vitiligo |
| Received:March 16, 2021 Revised:July 13, 2021 Click here to download the full text |
| Citation of this paper:LI Yi-lei,GAO Xing-hua,YANG Qiao-rong,XU Xin-zhi,YANG Ji,LI Ming.Proteomics study of differentially expressed proteins in patients with different stages of vitiligo[J].Chinese Journal of Clinical Medicine,2021,28(6):925-933 |
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| Author Name | Affiliation | E-mail | | LI Yi-lei | Department of Dermatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | GAO Xing-hua | Department of Dermatology, the First Hospital of China Medical University, Shenyang 110001, Liaoning, China | | | YANG Qiao-rong | Department of Dermatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | XU Xin-zhi | Department of Dermatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | YANG Ji | Department of Dermatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | LI Ming | Department of Dermatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | li.ming@zs-hospital.sh.cn |
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| Abstract:Objective: To identify and screen serum markers in different stages of vitiligo by 2 proteomic techniques, and to explore their relationship network.Methods: Serum samples were collected from 15 patients with vitiligo in stable stage, 15 patients with vitiligo in progressive stage, and 15 healthy individuals. Two-dimensional gel electrophoresis (2-DE) and isobaric tags for relative and absolute quantification (iTRAQ) were used to detect and label all samples. Differential proteins were screened, and the relationship of pathways was analyzed by software.Results: A total of 10 differential proteins were identified by 2-DE in patients with stable vitiligo, including 6 up-regulated and 4 down-regulated proteins; 25 differential proteins were identified in patients with progressive vitiligo, including 11 up-regulated and 14 down-regulated proteins. Six-two differential proteins (29 up-regulated and 33 down-regulated) in stable patients, and 50 differential proteins (30 up-regulated and 20 down-regulated) in advanced patients were identified by iTRAQ. The same differential proteins identified by the two proteomics include α-trypsin inhibitor heavy chain H4, complement C4-A (up-regulated) at the stable stage of vitiligo, and complement C4-B, apolipoprotein A (down-regulated) and α-trypsin inhibitor heavy chain H4 (up-regulated) at the advanced stage of vitiligo. According to GO annotation, the main pathways involved in the differential proteins were CCKR, plasminogen activator, p53, chemokine regulatory pathways, etc.Conclusions: The differentially expressed proteins in different stages of vitiligo could be screened by proteomic methods of both 2-DE and iTRAQ technology, which provided a foundation for further study of the pathogenesis of vitiligo. |
| keywords:two-dimensional gel electrophoresis (2-DE) isobaric tags for relative and absolute quantification (iTRAQ) vitiligo stage differential proteins |
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