| Effect of trehalose on electrophysiological characteristics of cryopreserved hippocampal neurons |
| Received:July 23, 2020 Revised:October 08, 2020 Click here to download the full text |
| Citation of this paper:WANG Guo-xiang,LIU Juan,ZHOU Zhi-yuan,LIU Xu.Effect of trehalose on electrophysiological characteristics of cryopreserved hippocampal neurons[J].Chinese Journal of Clinical Medicine,2020,27(5):773-778 |
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| Author Name | Affiliation | E-mail | | WANG Guo-xiang | Institutes of Brain Science, Fudan University, Shanghai 200032, China | | | LIU Juan | Department of Radiotherapy, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | ZHOU Zhi-yuan | Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | LIU Xu | Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | liu.xu@zs-hospital.sh.cn |
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| Abstract:Objective: To observe the changes in the electrophysiological characteristics of primary hippocampal neurons after cryopreservation under different conditions. Methods: Primary hippocampal neurons cultured in vitro and hippocampal neurons recovered after being frozen in serum-free cryo-medium, DF12+10% DMSO cryo-medium, DF12+10% DMSO+trehalose cryo-medium on the day of culture. After culturing for 14 to 16 days, the action potential, miniature inhibitory postsynaptic current (mIPSC), and miniature excitatory postsynaptic current (mEPSC) of each group were recorded by patch-clamp. Results: Electrophysiological characteristics of hippocampal neurons cryopreserved in cryopreservation fluid containing trehalose were similar to those of unfrozen primary culture hippocampal neurons. However, neurons cryopreserved in serum-free cryo-medium had obvious Burst-like discharge after resuscitation (P<0.05), the amplitude of mIPSC was weakened (P<0.001); similarly, neurons cryopreserved in DF12+10% DMSO cryo-medium also had obvious Burst-like discharge (P<0.05), the amplitude of mIPSC was weakened (P<0.001), and the frequency of mIPSC decreased (P<0.01). Conclusions: Trehalose has a protective effect on the frozen hippocampal neurons after resuscitation. |
| keywords:hippocampus neuron cryo-medium trehalose |
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