| Etiological dignostic value of metagenomic next-generation sequencing in spinal infection |
| Received:April 28, 2020 Revised:May 23, 2020 Click here to download the full text |
| Citation of this paper:JIN Wen-ting,LI Na,ZHOU Xiao-gang,ZHANG Yao,HUANG Ying-nan,SU Yi,YAO Yu-meng,MIAO Qing,MA Yu-yan,LI Bing,WANG Qing-qing,WANG Meng-ran,LUO Yu,CAI Si-shi,PAN Jue,HU Bi-jie.Etiological dignostic value of metagenomic next-generation sequencing in spinal infection[J].Chinese Journal of Clinical Medicine,2020,27(4):567-571 |
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| Author Name | Affiliation | E-mail | | JIN Wen-ting | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | LI Na | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | ZHOU Xiao-gang | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | ZHANG Yao | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | HUANG Ying-nan | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | SU Yi | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | YAO Yu-meng | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | MIAO Qing | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | MA Yu-yan | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | LI Bing | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | WANG Qing-qing | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | WANG Meng-ran | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | LUO Yu | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | CAI Si-shi | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | PAN Jue | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | pan.jue@zs-hospital.sh.cn | | HU Bi-jie | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | hu.bijie@zs-hospital.sh.cn |
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| Abstract:Objective: To explore the value of metagenomic next-generation sequencing (mNGS) in the pathogen diagnosis of spinal infection. Methods: A retrospective analysis was performed in 24 hospitalized patients with spinal infection in Zhongshan Hospital, Fudan University from January 2018 to December 2019. Spinal tissues were detected by microbial culture method and mNGS. Results: The overall positive rate of mNGS was higher than that of culture method (62.5% vs 35.0%). The average detection time of mNGS was 36-48 h, significantly shorter than that of culture method (21.8 days). The main pathogens of spinal infection were Mycobacterium tuberculosis complex, Staphylococcus aureus, and Escherichia coli. Conclusions: The mNGS is effective in quick detection of spinal infection pathogens, which can provide important etiological basis for early precise treatment. |
| keywords:spinal infection metagenomic next-generation sequencing microbiological diagnosis |
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