| Etiological diagnostic value of metagenomic next-generation sequencing in non-tuberculous mycobacteria infection |
| Received:June 04, 2020 Revised:August 19, 2020 Click here to download the full text |
| Citation of this paper:MIAO Qing,YAO Yu-meng,PAN Jue,BAO Rong,WANG Qing-qing,LI Na,CHEN Yan-qiong,JIN Wen-ting,ZHANG Yao,SU Yi,MA Yu-yan,HUANG Ying-nan,HU Bi-jie.Etiological diagnostic value of metagenomic next-generation sequencing in non-tuberculous mycobacteria infection[J].Chinese Journal of Clinical Medicine,2020,27(4):559-562 |
| Hits: 2731 |
| Download times: 852 |
| Author Name | Affiliation | E-mail | | MIAO Qing | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | YAO Yu-meng | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | PAN Jue | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | BAO Rong | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | WANG Qing-qing | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | LI Na | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | CHEN Yan-qiong | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | JIN Wen-ting | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | ZHANG Yao | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | SU Yi | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | MA Yu-yan | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | HUANG Ying-nan | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | HU Bi-jie | Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China | hu.bijie@zs-hospital.sh.cn |
|
| Abstract:Objective: To explore the value of metagenomic next-generation sequencing (mNGS) in etiological diagnosis of patients with non-tuberculosis mycobacterium (NTM) infection. Methods: A total of 183 NTM infected patients admitted to the Department of Infectious Diseases of Zhongshan Hospital, Fudan University from September 2018 to December 2019 were selected retrospectively, and a total of 388 samples were collected. The differences in the detection rate of NTM pathogens were analyzed by conventional microbial culture method and mNGS technology and the subgroup analysis of different sample types were performed. Results: There was no significant difference of positive rate between culture method and mNGS (21.9% vs 24.7%). Positive rate of sputum was significantly higher detected by culture method than that by mNGS (56.6% vs 22.9%,P<0.000 1), whereas for tissue type, positive rate is lower using culture method than that using mNGS (7.8% vs 27.8%,P<0.000 1). In addition, positive result of mNGS decreased frequency of antimicrobial modulation compared with culture method. Conclusions: mNGS is a promising technique, but the sensitivity of NTM is low, it is necessary to improve this technology to make it better applied in clinic. |
| keywords:non-tuberculosis mycobacteria metagenomic next-generation sequencing conventional microbial culture |
| HTML View Full Text View/Add Comment Download reader |
|
|
|