| Effect of MitoKATP channel on protective effect of isoflurane on acute lung injury |
| Received:March 17, 2020 Revised:May 15, 2020 Click here to download the full text |
| Citation of this paper:HAN Xiao-dan,SUN Min-li.Effect of MitoKATP channel on protective effect of isoflurane on acute lung injury[J].Chinese Journal of Clinical Medicine,2020,27(3):510-514 |
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| Abstract:Objective: To explore the effects of MitoKATP channel on the protective effect of isoflurane pretreatment on acute lung injury induced by endotoxin in piglets. Methods: Thirty young piglets of 5-6 weeks old, weighing 9-14 kg were anesthetized, intratracheally intubated, and underwent mechanical ventilation. The animals were divided into 5 groups (n=6 each group):control group, lipopolysaccharide (LPS) group, isoflurane (ISO) group, 5-hydroxydeconate (HD) group, and HD-ISO group. Control group was pretreated by normal saline, and LPS group was pretreated by endotoxin. ISO group was pretreated by alveolar concentrations (MAC) isoflurane, HD group was pretreated by 5-HD, HD-ISO group was pretreated by 5-HD, isoflurane, each group was pretreated and then injected endotoxin. After the establishment of acute lung injury model, the animals were killed and lungs were processed for measurement of peripheral white blood cell (WBC), the interleukin (IL) -8 and intercellular cell adhesion molecule (ICAM)-1 mRNA level, total phospholipid content in bronchoalveolar lavage fluid (BALF). Results: Compared with control group, WBC was decreased at ALI 0 h, 2 h, and 4 h in LPS group after ALI induction, IL-8 mRNA and ICAM-1 mRNA were increased, the total phospholipid content in BALF was decreased (P<0.05), hematoxylin-eosin (HE) staining showed that there was significant inflammatory cell infiltration in the lung tissue. Compared with LPS group, WBC was increased at 4 h in ISO group after ALI induction, IL-8 mRNA and ICAM-1 mRNA were decreased, the total phospholipid content in BALF was increased (P<0.05), HE staining showed less inflammatory cellular infiltration. Compared with ISO group, WBC was decreased 2 h and 4 h in HD-ISO group after ALI induction, IL-8 mRNA and ICAM-1 mRNA were increased, the total phospholipid content in BALF was decreased (P<0.05), H-E staining showed more inflammatory cell infiltration. Conclusions: Inhalation of isoflurane may inhibit the release of inflammatory factors and cell adhesion factors in piglets with acute lung injury through MitoKATP channel. |
| keywords:isoflurane MitoKATP channel acute lung injury IL-8 ICAM-1 |
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